Single-molecule localization microscopy reveals molecular transactions during RAD51 filament assembly at cellular DNA damage sites.
Haas, Kalina T
Nucleic acids research
Oxford University Press
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Haas, K. T., Lee, M., Esposito, A., & Venkitaraman, A. (2018). Single-molecule localization microscopy reveals molecular transactions during RAD51 filament assembly at cellular DNA damage sites.. Nucleic acids research, 46 (5), 2398-2416. https://doi.org/10.1093/nar/gkx1303
RAD51 recombinase assembles on single-stranded (ss)DNA substrates exposed by DNA end-resection to initiate homologous recombination (HR), a process fundamental to genome integrity. RAD51 assembly has been characterized using purified proteins, but its ultrastructural topography in the cell nucleus is unexplored. Here, we combine cell genetics with single-molecule localization microscopy and a palette of bespoke analytical tools, to visualize molecular transactions during RAD51 assembly in the cellular milieu at resolutions approaching 30–40 nm. In several human cell types, RAD51 focalizes in clusters that progressively extend into long filaments, which abut—but do not overlap—with globular bundles of replication protein A (RPA). Extended filaments alter topographically over time, suggestive of succeeding steps in HR. In cells depleted of the tumor suppressor protein BRCA2, or overexpressing its RAD51-binding BRC repeats, RAD51 fails to assemble at damage sites, although RPA accumulates unhindered. By contrast, in cells lacking a BRCA2 carboxyl (C)-terminal region targeted by cancer-causing mutations, damage-induced RAD51 assemblies initiate but do not extend into filaments. We suggest a model wherein RAD51 assembly proceeds concurrently with end-resection at adjacent sites, via an initiation step dependent on the BRC repeats, followed by filament extension through the C-terminal region of BRCA2.
Cell Line, Hela Cells, Humans, DNA Damage, BRCA2 Protein, DNA, Single-Stranded, Microscopy, DNA Repair, Kinetics, Rad51 Recombinase, Replication Protein A
Wellcome Trust (090340/Z/09/Z)
External DOI: https://doi.org/10.1093/nar/gkx1303
This record's URL: https://www.repository.cam.ac.uk/handle/1810/277421
Attribution 4.0 International
Licence URL: http://creativecommons.org/licenses/by/4.0/
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