Preterm Infant-Associated Clostridium tertium, Clostridium cadaveris, and Clostridium paraputrificum Strains: Genomic and Evolutionary Insights.
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Authors
Kiu, Raymond
Caim, Shabhonam
Alcon-Giner, Cristina
Belteki, Gusztav
Clarke, Paul
Pickard, Derek
Hall, Lindsay J
Publication Date
2017-10-01Journal Title
Genome Biol Evol
ISSN
1759-6653
Publisher
Oxford University Press (OUP)
Volume
9
Issue
10
Pages
2707-2714
Language
eng
Type
Article
This Version
VoR
Physical Medium
Print
Metadata
Show full item recordCitation
Kiu, R., Caim, S., Alcon-Giner, C., Belteki, G., Clarke, P., Pickard, D., Dougan, G., & et al. (2017). Preterm Infant-Associated Clostridium tertium, Clostridium cadaveris, and Clostridium paraputrificum Strains: Genomic and Evolutionary Insights.. Genome Biol Evol, 9 (10), 2707-2714. https://doi.org/10.1093/gbe/evx210
Abstract
Clostridium species (particularly Clostridium difficile, Clostridium botulinum, Clostridium tetani and Clostridium perfringens) are associated with a range of human and animal diseases. Several other species including Clostridium tertium, Clostridium cadaveris, and Clostridium paraputrificum have also been linked with sporadic human infections, however there is very limited, or in some cases, no genomic information publicly available. Thus, we isolated one C. tertium strain, one C. cadaveris strain and three C. paraputrificum strains from preterm infants residing within neonatal intensive care units and performed Whole Genome Sequencing (WGS) using Illumina HiSeq. In this report, we announce the open availability of the draft genomes: C. tertium LH009, C. cadaveris LH052, C. paraputrificum LH025, C. paraputrificum LH058, and C. paraputrificum LH141. These genomes were checked for contamination in silico to ensure purity, and we confirmed species identity and phylogeny using both 16S rRNA gene sequences (from PCR and in silico) and WGS-based approaches. Average Nucleotide Identity (ANI) was used to differentiate genomes from their closest relatives to further confirm speciation boundaries. We also analysed the genomes for virulence-related factors and antimicrobial resistance genes, and detected presence of tetracycline and methicillin resistance, and potentially harmful enzymes, including multiple phospholipases and toxins. The availability of genomic data in open databases, in tandem with our initial insights into the genomic content and virulence traits of these pathogenic Clostridium species, should enable the scientific community to further investigate the disease-causing mechanisms of these bacteria with a view to enhancing clinical diagnosis and treatment.
Keywords
Feces, Humans, Clostridium, Clostridium tertium, Phylogeny, Genome, Bacterial, Infant, Newborn, Infant, Premature, Intensive Care Units, Neonatal
Sponsorship
Cambridge University Hospitals NHS Foundation Trust (CUH) (BRC)
Wellcome Trust (100974/B/13/Z)
Identifiers
External DOI: https://doi.org/10.1093/gbe/evx210
This record's URL: https://www.repository.cam.ac.uk/handle/1810/277422
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