Massive crossover elevation via combination of HEI10 and recq4a recq4b during Arabidopsis meiosis.
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Authors
Publication Date
2018-03-06Journal Title
Proceedings of the National Academy of Sciences of the United States of America
ISSN
1091-6490
Publisher
National Academy of Sciences
Volume
115
Issue
10
Pages
2437-2442
Type
Article
This Version
VoR
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Henderson, I. (2018). Massive crossover elevation via combination of HEI10 and recq4a recq4b during Arabidopsis meiosis.. Proceedings of the National Academy of Sciences of the United States of America, 115 (10), 2437-2442. https://doi.org/10.1073/pnas.1713071115
Abstract
During meiosis, homologous chromosomes undergo reciprocal
crossovers, which generate genetic diversity and underpin classical
crop improvement. Meiotic recombination initiates from DNA
double-strand breaks (DSBs), which are processed into singlestranded
DNA that can invade a homologous chromosome. The
resulting joint molecules can ultimately be resolved as crossovers. In
Arabidopsis, competing pathways balance the repair of ∼100–
200 meiotic DSBs into ∼10 crossovers per meiosis, with the excess
DSBs repaired as noncrossovers. To bias DSB repair toward crossovers,
we simultaneously increased dosage of the procrossover E3 ligase
gene HEI10 and introduced mutations in the anticrossovers helicase
genes RECQ4A and RECQ4B. As HEI10 and recq4a recq4b increase interfering
and noninterfering crossover pathways, respectively, they
combine additively to yield a massive meiotic recombination increase.
Interestingly, we also show that increased HEI10 dosage increases
crossover coincidence, which indicates an effect on interference. We
also show that patterns of interhomolog polymorphism and heterochromatin
drive recombination increases distally towards the subtelomeres
in both HEI10 and recq4a recq4b backgrounds, while the
centromeres remain crossover suppressed. These results provide a
genetic framework for engineering meiotic recombination landscapes
in plant genomes.
Keywords
meiosis, crossover, recombination, HEI10, RECQ4
Sponsorship
Biotechnology and Biological Sciences Research Council (BBSRC) grant BB/L006847/1, BBSRC-Meiogenix IPA grant BB/N007557/1, European Research Area Network for Coodinating Action in Plant Sciences/BBSRC “DeCOP” (BB/M004937/1), Marie-Curie “COMREC” network FP7
ITN-606956, European Research Council (SynthHotspot CoG) and Gatsby Charitable Foundation (GAT2962), a Royal Society University Research Fellowship, and the Bettencourt Schueller Foundation.
Funder references
The Royal Society (uf0762030)
Isaac Newton Trust (1026(ab))
Gatsby Charitable Foundation (GAT2962)
Biotechnology and Biological Sciences Research Council (BB/L006847/1)
Biotechnology and Biological Sciences Research Council (BB/M004937/1)
The Royal Society (uf120133)
European Commission (606956)
Gatsby Charitable Foundation (GAT3401)
Biotechnology and Biological Sciences Research Council (BB/N007557/1)
European Research Council (681987)
European Research Council (790445)
Biotechnology and Biological Sciences Research Council (BB/K007882/1)
Identifiers
External DOI: https://doi.org/10.1073/pnas.1713071115
This record's URL: https://www.repository.cam.ac.uk/handle/1810/278452
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