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dc.contributor.authorJedrey, Hannahen
dc.contributor.authorLilley, Kathrynen
dc.contributor.authorWelch, Martinen
dc.date.accessioned2018-08-08T13:38:53Z
dc.date.available2018-08-08T13:38:53Z
dc.date.issued2018-07en
dc.identifier.issn0378-1097
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/278703
dc.description.abstractCiprofloxacin is one of the most widely-used antibiotics, and has proven especially effective at controlling infections associated with the opportunistic human pathogen, Pseudomonas aeruginosa. In this work, we show that sub-inhibitory concentrations of ciprofloxacin induce discrete changes in the intracellular proteome. Central metabolism and cell envelope-associated functions are particularly affected. In spite of the low magnitude of the intracellular proteomic changes, we found that sub-lethal concentrations of ciprofloxacin had substantial effects on motility and exoprotein secretion. Crucially, the proteomic and phenotypic modulations that we observed were absolutely dependent upon the presence of wild-type GyrA; an isogenic strain of P. aeruginosa carrying a ciprofloxacin-insensitive form of GyrA (a T83→I mutant) did not display ciprofloxacin-dependent changes unless complemented with wild-type gyrA in trans. These results show that the diverse effects of sub-inhibitory ciprofloxacin on the cell are routed through its primary target in the cell, DNA gyrase.
dc.description.sponsorshipThis work was supported by BBSRC grants BB/C500252/1 and BB/M019411/1.
dc.format.mediumPrinten
dc.languageengen
dc.publisherOUP
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectPseudomonas aeruginosaen
dc.subjectCiprofloxacinen
dc.subjectDNA Gyraseen
dc.subjectBacterial Proteinsen
dc.subjectProteomeen
dc.subjectAnti-Bacterial Agentsen
dc.subjectMicrobial Sensitivity Testsen
dc.subjectMutationen
dc.titleCiprofloxacin binding to GyrA causes global changes in the proteome of Pseudomonas aeruginosa.en
dc.typeArticle
prism.issueIdentifier13en
prism.publicationDate2018en
prism.publicationNameFEMS microbiology lettersen
prism.volume365en
dc.identifier.doi10.17863/CAM.22505
dcterms.dateAccepted2018-05-25en
rioxxterms.versionofrecord10.1093/femsle/fny134en
rioxxterms.versionVoR*
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2018-07en
dc.contributor.orcidLilley, Kathryn [0000-0003-0594-6543]
dc.contributor.orcidWelch, Martin [0000-0003-3646-1733]
dc.identifier.eissn1574-6968
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idBBSRC (BB/C500252/1)
pubs.funder-project-idBBSRC (BB/M019411/1)
cam.orpheus.successThu Jan 30 13:00:11 GMT 2020 - The item has an open VoR version.*
rioxxterms.freetoread.startdate2100-01-01


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International