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dc.contributor.authorBaird, Arabellaen
dc.contributor.authorLindsay, Timothyen
dc.contributor.authorEverett, Aliceen
dc.contributor.authorIyemere, Valentineen
dc.contributor.authorPaterson, Yasminen
dc.contributor.authorMcClellan, Alyceen
dc.contributor.authorHenson, Francesen
dc.contributor.authorGuest, Deborah Jen
dc.date.accessioned2018-08-17T16:41:11Z
dc.date.available2018-08-17T16:41:11Z
dc.date.issued2018-05-10en
dc.identifier.issn2046-6390
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/278949
dc.description.abstractBone fractures occur in horses following traumatic and non-traumatic (bone overloading) events. They can be difficult to treat due to the need for the horse to bear weight on all legs during the healing period. Regenerative medicine to improve fracture union and recovery could significantly improve horse welfare.Equine induced pluripotent stem cells (iPSCs) have previously been derived. Here we show that equine iPSCs cultured for 21 days in osteogenic induction media on an OsteoAssay surface upregulate the expression of osteoblast associated genes and proteins, including COL1A1, SPARC, SPP1, IBSP, RUNX2 and BGALPWe also demonstrate that iPSC-osteoblasts are able to produce a mineralised matrix with both calcium and hydroxyapatite deposition. Alkaline phosphatase activity is also significantly increased during osteoblast differentiation.Although the genetic background of the iPSC donor animal affects the level of differentiation observed after 21 days of differentiation, less variation between lines of iPSCs derived from the same horse was observed.The successful, direct, differentiation of equine iPSCs into osteoblasts may provide a source of cells for future regenerative medicine strategies to improve fracture repair in horses undergoing surgery. iPSC-derived osteoblasts will also provide a potential tool to study equine bone development and disease.
dc.description.sponsorshipAnne Duchess of Cambridge Charitable Trust, Paul Mellon Foundation, Cambridge Turst
dc.format.mediumElectronicen
dc.languageengen
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleOsteoblast differentiation of equine induced pluripotent stem cells.en
dc.typeArticle
prism.issueIdentifier5en
prism.publicationDate2018en
prism.publicationNameBiology openen
prism.volume7en
dc.identifier.doi10.17863/CAM.26334
dcterms.dateAccepted2018-04-15en
rioxxterms.versionofrecord10.1242/bio.033514en
rioxxterms.versionVoR*
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2018-05-10en
dc.contributor.orcidBaird, Arabella [0000-0003-2689-1481]
dc.contributor.orcidLindsay, Timothy [0000-0003-2857-1764]
dc.contributor.orcidEverett, Alice [0000-0002-1679-3936]
dc.contributor.orcidHenson, Frances [0000-0002-3518-1492]
dc.contributor.orcidGuest, Deborah J [0000-0002-0034-3332]
dc.identifier.eissn2046-6390
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idMRC (MC_PC_12009)


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International