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dc.contributor.authorShishmarev, Dmitry
dc.contributor.authorWright, Alan
dc.contributor.authorRodrigues, Tiago B
dc.contributor.authorPileio, Giuseppe
dc.contributor.authorStevanato, Gabriele
dc.contributor.authorBrindle, Kevin
dc.contributor.authorKuchel, Philip W
dc.date.accessioned2018-09-04T08:54:04Z
dc.date.available2018-09-04T08:54:04Z
dc.date.issued2018-03
dc.identifier.issn1099-1492
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/279068
dc.description.abstractFumarate is an important probe of metabolism in hyperpolarized magnetic resonance imaging and spectroscopy. It is used to detect the release of fumarase in cancer tissues, which is associated with necrosis and drug treatment. Nevertheless, there are limited reports describing the detailed kinetic studies of this enzyme in various cells and tissues. Thus, we aimed to evaluate the sub-minute kinetics of human red blood cell fumarase using nuclear magnetic resonance (NMR) spectroscopy, and to provide a quantitative description of the enzyme that is relevant to the use of fumarate as a probe of cell rupture. The fumarase reaction was studied using time courses of 1 H spin-echo and 13 C-NMR spectra. 1 H-NMR experiments showed that the fumarase reaction in hemolysates is sufficiently rapid to make its kinetics amenable to study in a period of approximately 3 min, a timescale characteristic of hyperpolarized 13 C-NMR spectroscopy. The rapid-dissolution dynamic nuclear polarization (RD-DNP) technique was used to hyperpolarize [1,4-13 C]fumarate, which was injected into concentrated hemolysates. The kinetic data were analyzed using recently developed FmRα analysis and modeling of the enzymatic reaction using Michaelis-Menten equations. In RD-DNP experiments, the decline in the 13 C-NMR signal from fumarate, and the concurrent rise and fall of that from malate, were captured with high spectral resolution and signal-to-noise ratio, which allowed the robust quantification of fumarase kinetics. The kinetic parameters obtained indicate the potential contribution of hemolysis to the overall rate of the fumarase reaction when 13 C-NMR RD-DNP is used to detect necrosis in animal models of implanted tumors. The analytical procedures developed will be applicable to studies of other rapid enzymatic reactions using conventional and hyperpolarized substrate NMR spectroscopy.
dc.languageeng
dc.publisherWiley-Blackwell
dc.subjectMichaelis-Menten model
dc.subjectfumarase kinetics
dc.subjectfumarate
dc.subjecthuman red blood cell
dc.subjecthyperpolarization
dc.subjectmalate
dc.titleSub-minute kinetics of human red cell fumarase: 1 H spin-echo NMR spectroscopy and 13 C rapid-dissolution dynamic nuclear polarization.
dc.typeArticle
prism.issueIdentifier3
prism.numbere3870
prism.publicationDate2018
prism.publicationNameNMR in Biomedicine
prism.volume31
dc.identifier.doi10.17863/CAM.26450
dcterms.dateAccepted2017-10-30
rioxxterms.versionofrecord10.1002/nbm.3870
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-03
dc.contributor.orcidWright, Alan [0000-0002-4577-5681]
dc.contributor.orcidBrindle, Kevin [0000-0003-3883-6287]
dc.identifier.eissn1099-1492
dc.publisher.urlhttps://onlinelibrary.wiley.com/doi/abs/10.1002/nbm.3870
rioxxterms.typeJournal Article/Review
cam.issuedOnline2018-01-08
dc.identifier.urlhttps://onlinelibrary.wiley.com/doi/abs/10.1002/nbm.3870
rioxxterms.freetoread.startdate2019-01-08


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