Imaging the Effector CD8 Synapse.
Accepted version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Frazer, Gordon L
Asano, Yukako
Griffiths, Gillian M
Abstract
Here, we describe 4D imaging of effector CD8+ T cells as they conjugate and kill live targets in vitro and analyze the polarization dynamics of intracellular compartments to this cell-cell interface.
Description
Keywords
4D object-based image analysis, Imaris, Nucleofection, Time-lapse confocal imaging, Animals, CD8-Positive T-Lymphocytes, Humans, Imaging, Three-Dimensional, Immunological Synapses, Microscopy, Confocal
Journal Title
Methods Mol Biol
Conference Name
Journal ISSN
1064-3745
1940-6029
1940-6029
Volume Title
1584
Publisher
Springer New York
Publisher DOI
Sponsorship
Wellcome Trust (103930/Z/14/Z)