An Iterative Module in the Azalomycin F Polyketide Synthase Contains a Switchable Enoylreductase Domain.
dc.contributor.author | Xu, Wei | |
dc.contributor.author | Zhai, Guifa | |
dc.contributor.author | Liu, Yuanzhen | |
dc.contributor.author | Li, Yuan | |
dc.contributor.author | Shi, Yanrong | |
dc.contributor.author | Hong, Kui | |
dc.contributor.author | Hong, Hui | |
dc.contributor.author | Leadlay, Peter F | |
dc.contributor.author | Deng, Zixin | |
dc.contributor.author | Sun, Yuhui | |
dc.date.accessioned | 2018-09-05T12:47:46Z | |
dc.date.available | 2018-09-05T12:47:46Z | |
dc.date.issued | 2017-05-08 | |
dc.identifier.issn | 1433-7851 | |
dc.identifier.uri | https://www.repository.cam.ac.uk/handle/1810/279548 | |
dc.description.abstract | Detailed analysis of the modular Type I polyketide synthase (PKS) involved in the biosynthesis of the marginolactone azalomycin F in mangrove Streptomyces sp. 211726 has shown that only nineteen extension modules are required to accomplish twenty cycles of polyketide chain elongation. Analysis of the products of a PKS mutant specifically inactivated in the dehydratase domain of extension-module 1 showed that this module catalyzes two successive elongations with different outcomes. Strikingly, the enoylreductase domain of this module can apparently be "toggled" off and on : it functions in only the second of these two cycles. This novel mechanism expands our understanding of PKS assembly-line catalysis and may explain examples of apparent non-colinearity in other modular PKS systems. | |
dc.format.medium | Print-Electronic | |
dc.language | eng | |
dc.publisher | Wiley | |
dc.rights | Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) | |
dc.subject | Macrolides | |
dc.subject | Polyketide Synthases | |
dc.subject | Oxidoreductases | |
dc.subject | Molecular Conformation | |
dc.subject | Mutation | |
dc.title | An Iterative Module in the Azalomycin F Polyketide Synthase Contains a Switchable Enoylreductase Domain. | |
dc.type | Article | |
prism.endingPage | 5506 | |
prism.issueIdentifier | 20 | |
prism.publicationDate | 2017 | |
prism.publicationName | Angew Chem Int Ed Engl | |
prism.startingPage | 5503 | |
prism.volume | 56 | |
dc.identifier.doi | 10.17863/CAM.26920 | |
rioxxterms.versionofrecord | 10.1002/anie.201701220 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.licenseref.startdate | 2017-05 | |
dc.contributor.orcid | Leadlay, Peter [0000-0002-3247-509X] | |
dc.identifier.eissn | 1521-3773 | |
rioxxterms.type | Journal Article/Review | |
pubs.funder-project-id | Biotechnology and Biological Sciences Research Council (BB/I002413/1) | |
cam.issuedOnline | 2017-04-18 |
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