Show simple item record

dc.contributor.authorSowton, Aliceen
dc.contributor.authorMillington-Burgess, Sarah Len
dc.contributor.authorMurray, Andrewen
dc.contributor.authorHarper, Matthewen
dc.date.accessioned2018-09-05T12:51:18Z
dc.date.available2018-09-05T12:51:18Z
dc.date.issued2018-09en
dc.identifier.issn0006-291X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/279645
dc.description.abstractPlatelet activation plays a key role in normal haemostasis and pathological thrombosis. Platelet activation is rapid; within minutes of stimulation, platelets generate bioactive phospholipids, secrete their granule contents, activate integrins and aggregate together to form a haemostatic plug. These events are dependent on ATP synthesis. Mitochondrial function in platelets from healthy volunteers and patients with a range of diseases indicate an important role for oxygen consumption in oxidative phosphorylation in normal and pathological function. Platelets also consume oxygen during oxidation reactions, such as cyclooxygenase-dependent thromboxane A2 synthesis. In this study, we used high-resolution respirometry to investigate rapid changes in oxygen consumption during platelet activation. We demonstrated a rapid, transient increase in oxygen consumption rate within minutes of platelet stimulation by the physiological activator, thrombin. This was partly inhibited by aspirin and by oligomycin. This shows that high resolution respirometry can provide information regarding rapid and dynamic changes in oxygen consumption during platelet activation.
dc.format.mediumPrint-Electronicen
dc.languageengen
dc.publisherElsevier
dc.rightsAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBlood Plateletsen
dc.subjectHumansen
dc.subjectOxygenen
dc.subjectAspirinen
dc.subjectOligomycinsen
dc.subjectThrombinen
dc.subjectCell Respirationen
dc.subjectOxygen Consumptionen
dc.subjectPlatelet Aggregationen
dc.subjectKineticsen
dc.subjectPrimary Cell Cultureen
dc.titleRapid kinetics of changes in oxygen consumption rate in thrombin-stimulated platelets measured by high-resolution respirometry.en
dc.typeArticle
prism.endingPage2727
prism.issueIdentifier4en
prism.publicationDate2018en
prism.publicationNameBiochemical and biophysical research communicationsen
prism.startingPage2721
prism.volume503en
dc.identifier.doi10.17863/CAM.27013
dcterms.dateAccepted2018-08-03en
rioxxterms.versionofrecord10.1016/j.bbrc.2018.08.031en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2018-09en
dc.contributor.orcidSowton, Alice [0000-0002-3718-7783]
dc.contributor.orcidMurray, Andrew [0000-0002-0929-9315]
dc.contributor.orcidHarper, Matthew [0000-0002-4740-637X]
dc.identifier.eissn1090-2104
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idIsaac Newton Trust (1044(y))


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY 4.0)