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dc.contributor.authorSowton, Alice
dc.contributor.authorMillington-Burgess, Sarah L
dc.contributor.authorMurray, Andrew
dc.contributor.authorHarper, Matthew
dc.date.accessioned2018-09-05T12:51:18Z
dc.date.available2018-09-05T12:51:18Z
dc.date.issued2018-09-18
dc.identifier.issn0006-291X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/279645
dc.description.abstractPlatelet activation plays a key role in normal haemostasis and pathological thrombosis. Platelet activation is rapid; within minutes of stimulation, platelets generate bioactive phospholipids, secrete their granule contents, activate integrins and aggregate together to form a haemostatic plug. These events are dependent on ATP synthesis. Mitochondrial function in platelets from healthy volunteers and patients with a range of diseases indicate an important role for oxygen consumption in oxidative phosphorylation in normal and pathological function. Platelets also consume oxygen during oxidation reactions, such as cyclooxygenase-dependent thromboxane A2 synthesis. In this study, we used high-resolution respirometry to investigate rapid changes in oxygen consumption during platelet activation. We demonstrated a rapid, transient increase in oxygen consumption rate within minutes of platelet stimulation by the physiological activator, thrombin. This was partly inhibited by aspirin and by oligomycin. This shows that high resolution respirometry can provide information regarding rapid and dynamic changes in oxygen consumption during platelet activation.
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherElsevier BV
dc.rightsAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBlood Platelets
dc.subjectHumans
dc.subjectOxygen
dc.subjectAspirin
dc.subjectOligomycins
dc.subjectThrombin
dc.subjectCell Respiration
dc.subjectOxygen Consumption
dc.subjectPlatelet Aggregation
dc.subjectKinetics
dc.subjectPrimary Cell Culture
dc.titleRapid kinetics of changes in oxygen consumption rate in thrombin-stimulated platelets measured by high-resolution respirometry.
dc.typeArticle
prism.endingPage2727
prism.issueIdentifier4
prism.publicationDate2018
prism.publicationNameBiochem Biophys Res Commun
prism.startingPage2721
prism.volume503
dc.identifier.doi10.17863/CAM.27013
dcterms.dateAccepted2018-08-03
rioxxterms.versionofrecord10.1016/j.bbrc.2018.08.031
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-09
dc.contributor.orcidSowton, Alice [0000-0002-3718-7783]
dc.contributor.orcidMurray, Andrew [0000-0002-0929-9315]
dc.contributor.orcidHarper, Matthew [0000-0002-4740-637X]
dc.identifier.eissn1090-2104
rioxxterms.typeJournal Article/Review
pubs.funder-project-idIsaac Newton Trust (1044(y))


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY 4.0)