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dc.contributor.authorEves-van den Akker, Sebastian
dc.contributor.authorLilley, Catherine J
dc.contributor.authorJones, John T
dc.contributor.authorUrwin, Peter E
dc.date.accessioned2018-09-08T06:30:24Z
dc.date.available2018-09-08T06:30:24Z
dc.date.issued2014-09
dc.identifier.issn1553-7366
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/279787
dc.description.abstractSedentary endoparasitic nematodes are obligate biotrophs that modify host root tissues, using a suite of effector proteins to create and maintain a feeding site that is their sole source of nutrition. Using assumptions about the characteristics of genes involved in plant-nematode biotrophic interactions to inform the identification strategy, we provide a description and characterisation of a novel group of hyper-variable extracellular effectors termed HYP, from the potato cyst nematode Globodera pallida. HYP effectors comprise a large gene family, with a modular structure, and have unparalleled diversity between individuals of the same population: no two nematodes tested had the same genetic complement of HYP effectors. Individuals vary in the number, size, and type of effector subfamilies. HYP effectors are expressed throughout the biotrophic stages in large secretory cells associated with the amphids of parasitic stage nematodes as confirmed by in situ hybridisation. The encoded proteins are secreted into the host roots where they are detectable by immunochemistry in the apoplasm, between the anterior end of the nematode and the feeding site. We have identified HYP effectors in three genera of plant parasitic nematodes capable of infecting a broad range of mono- and dicotyledon crop species. In planta RNAi targeted to all members of the effector family causes a reduction in successful parasitism.
dc.format.mediumElectronic-eCollection
dc.languageeng
dc.publisherPublic Library of Science (PLoS)
dc.rightsAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCell Wall
dc.subjectAnimals
dc.subjectTylenchoidea
dc.subjectPlant Roots
dc.subjectSecernentea Infections
dc.subjectHelminth Proteins
dc.subjectDNA, Helminth
dc.subjectImmunoblotting
dc.subjectIn Situ Hybridization
dc.subjectCloning, Molecular
dc.subjectComputational Biology
dc.subjectPlant Diseases
dc.subjectAmino Acid Sequence
dc.subjectSequence Homology, Amino Acid
dc.subjectLife Cycle Stages
dc.subjectMultigene Family
dc.subjectMolecular Sequence Data
dc.subjectSolanum tuberosum
dc.subjectHost-Parasite Interactions
dc.subjectDNA Copy Number Variations
dc.subjectHigh-Throughput Nucleotide Sequencing
dc.subjectPlant Cells
dc.titleIdentification and characterisation of a hyper-variable apoplastic effector gene family of the potato cyst nematodes.
dc.typeArticle
prism.issueIdentifier9
prism.publicationDate2014
prism.publicationNamePLoS Pathog
prism.startingPagee1004391
prism.volume10
dc.identifier.doi10.17863/CAM.27157
dcterms.dateAccepted2014-08-11
rioxxterms.versionofrecord10.1371/journal.ppat.1004391
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2014-09-25
dc.contributor.orcidEves-Van Den Akker, Sebastian [0000-0002-8833-9679]
dc.identifier.eissn1553-7374
rioxxterms.typeJournal Article/Review
cam.issuedOnline2014-09-25


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY 4.0)