Rapid flow cytometric measurement of protein inclusions and nuclear trafficking.
San Gil, R
Springer Science and Business Media LLC
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Whiten, D., San Gil, R., McAlary, L., Yerbury, J., Ecroyd, H., & Wilson, M. (2016). Rapid flow cytometric measurement of protein inclusions and nuclear trafficking.. Sci Rep, 6 31138. https://doi.org/10.1038/srep31138
Proteinaceous cytoplasmic inclusions are an indicator of dysfunction in normal cellular proteostasis and a hallmark of many neurodegenerative diseases. We describe a simple and rapid new flow cytometry-based method to enumerate, characterise and, if desired, physically recover protein inclusions from cells. This technique can analyse and resolve a broad variety of inclusions differing in both size and protein composition, making it applicable to essentially any model of intracellular protein aggregation. The method also allows rapid quantification of the nuclear trafficking of fluorescently labelled molecules.
Cell Line, Inclusion Bodies, Cell Nucleus, Humans, Neurodegenerative Diseases, Proteins, Calibration, Flow Cytometry, Protein Transport
External DOI: https://doi.org/10.1038/srep31138
This record's URL: https://www.repository.cam.ac.uk/handle/1810/279805
Attribution 4.0 International (CC BY 4.0)
Licence URL: https://creativecommons.org/licenses/by/4.0/