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dc.contributor.authorWhiten, Daniel
dc.contributor.authorSan Gil, R
dc.contributor.authorMcAlary, L
dc.contributor.authorYerbury, JJ
dc.contributor.authorEcroyd, H
dc.contributor.authorWilson, MR
dc.date.accessioned2018-09-08T06:30:58Z
dc.date.available2018-09-08T06:30:58Z
dc.date.issued2016-08-12
dc.identifier.issn2045-2322
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/279805
dc.description.abstractProteinaceous cytoplasmic inclusions are an indicator of dysfunction in normal cellular proteostasis and a hallmark of many neurodegenerative diseases. We describe a simple and rapid new flow cytometry-based method to enumerate, characterise and, if desired, physically recover protein inclusions from cells. This technique can analyse and resolve a broad variety of inclusions differing in both size and protein composition, making it applicable to essentially any model of intracellular protein aggregation. The method also allows rapid quantification of the nuclear trafficking of fluorescently labelled molecules.
dc.format.mediumElectronic
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.rightsAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCell Line
dc.subjectInclusion Bodies
dc.subjectCell Nucleus
dc.subjectHumans
dc.subjectNeurodegenerative Diseases
dc.subjectProteins
dc.subjectCalibration
dc.subjectFlow Cytometry
dc.subjectProtein Transport
dc.titleRapid flow cytometric measurement of protein inclusions and nuclear trafficking.
dc.typeArticle
prism.publicationDate2016
prism.publicationNameSci Rep
prism.startingPage31138
prism.volume6
dc.identifier.doi10.17863/CAM.27175
dcterms.dateAccepted2016-07-14
rioxxterms.versionofrecord10.1038/srep31138
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2016-08-12
dc.contributor.orcidWhiten, Daniel [0000-0002-7853-3566]
dc.identifier.eissn2045-2322
rioxxterms.typeJournal Article/Review
cam.issuedOnline2016-08-12


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY 4.0)