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dc.contributor.authorMorgan, Michael
dc.contributor.authorMarioni, John
dc.date.accessioned2018-09-08T06:33:46Z
dc.date.available2018-09-08T06:33:46Z
dc.date.issued2018-06-26
dc.identifier.issn1474-7596
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/279891
dc.description.abstractBACKGROUND: Population phenotypic variation can arise from genetic differences between individuals, or from cellular heterogeneity in an isogenic group of cells or organisms. The emergence of gene expression differences between genetically identical cells is referred to as gene expression noise, the sources of which are not well understood. RESULTS: In this work, by studying gene expression noise between multiple cell lineages and mammalian species, we find consistent evidence of a role for CpG islands as sources of gene expression noise. Variation in noise among CpG island promoters can be partially attributed to differences in island size, in which short islands have noisier gene expression. Building on these findings, we investigate the potential for short CpG islands to act as fast response elements to environmental stimuli. Specifically, we find that these islands are enriched amongst primary response genes in SWI/SNF-independent stimuli, suggesting that expression noise is an indicator of promoter responsiveness. CONCLUSIONS: Thus, through the integration of single-cell RNA expression profiling, chromatin landscape and temporal gene expression dynamics, we have uncovered a role for short CpG island promoters as fast response elements.
dc.format.mediumElectronic
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.rightsAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAnimals
dc.subjectMammals
dc.subjectMice
dc.subjectGene Expression
dc.subjectCpG Islands
dc.subjectCell Lineage
dc.subjectPromoter Regions, Genetic
dc.subjectGenetic Variation
dc.titleCpG island composition differences are a source of gene expression noise indicative of promoter responsiveness.
dc.typeArticle
prism.issueIdentifier1
prism.publicationDate2018
prism.publicationNameGenome Biol
prism.startingPage81
prism.volume19
dc.identifier.doi10.17863/CAM.27259
dcterms.dateAccepted2018-06-04
rioxxterms.versionofrecord10.1186/s13059-018-1461-x
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-06-26
dc.contributor.orcidMorgan, Michael [0000-0003-0757-0711]
dc.contributor.orcidMarioni, John [0000-0001-9092-0852]
dc.identifier.eissn1474-760X
rioxxterms.typeJournal Article/Review
pubs.funder-project-idCancer Research UK (C14303/A17197)
cam.issuedOnline2018-06-26


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY 4.0)