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dc.contributor.authorZaini, M Nazhif
dc.contributor.authorPatel, Saroor A
dc.contributor.authorSyafruddin, Saiful E
dc.contributor.authorRodrigues, Paulo
dc.contributor.authorVanharanta, Sakari
dc.date.accessioned2018-09-08T06:33:49Z
dc.date.available2018-09-08T06:33:49Z
dc.date.issued2018-08-13
dc.identifier.issn2045-2322
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/279893
dc.description.abstractTissue-specific transcriptional programs control most biological phenotypes, including disease states such as cancer. However, the molecular details underlying transcriptional specificity is largely unknown, hindering the development of therapeutic approaches. Here, we describe novel experimental reporter systems that allow interrogation of the endogenous expression of HIF2A, a critical driver of renal oncogenesis. Using a focused CRISPR-Cas9 library targeting chromatin regulators, we provide evidence that these reporter systems are compatible with high-throughput screening. Our data also suggests redundancy in the control of cancer type-specific transcriptional traits. Reporter systems such as those described here could facilitate large-scale mechanistic dissection of transcriptional programmes underlying cancer phenotypes, thus paving the way for novel therapeutic approaches.
dc.format.mediumElectronic
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.rightsAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCell Line, Tumor
dc.subjectHumans
dc.subjectCarcinoma, Renal Cell
dc.subjectKidney Neoplasms
dc.subjectFeasibility Studies
dc.subjectGene Expression Profiling
dc.subjectTranscription, Genetic
dc.subjectGene Expression Regulation, Neoplastic
dc.subjectGenes, Reporter
dc.subjectBasic Helix-Loop-Helix Transcription Factors
dc.subjectGene Regulatory Networks
dc.subjectGene Knock-In Techniques
dc.subjectGenetic Testing
dc.subjectHigh-Throughput Screening Assays
dc.subjectHEK293 Cells
dc.subjectCarcinogenesis
dc.subjectCRISPR-Cas Systems
dc.titleEndogenous HIF2A reporter systems for high-throughput functional screening.
dc.typeArticle
prism.issueIdentifier1
prism.publicationDate2018
prism.publicationNameSci Rep
prism.startingPage12063
prism.volume8
dc.identifier.doi10.17863/CAM.27261
dcterms.dateAccepted2018-08-01
rioxxterms.versionofrecord10.1038/s41598-018-30499-2
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-08-13
dc.contributor.orcidVanharanta, Sakari [0000-0001-5619-7963]
dc.identifier.eissn2045-2322
rioxxterms.typeJournal Article/Review
pubs.funder-project-idMedical Research Council (MC_UU_12022/7)
pubs.funder-project-idMRC (unknown)
pubs.funder-project-idMajlis Amanah Rakyat (MARA) (unknown)
pubs.funder-project-idUniversity of Malaya (UM) (UKM(PER)K019321)
pubs.funder-project-idMRC (MR/N501876/1)
cam.issuedOnline2018-08-13


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY 4.0)