Show simple item record

dc.contributor.authorZiv, Omeren
dc.contributor.authorGabryelska, Marta Men
dc.contributor.authorLun, Aaronen
dc.contributor.authorGebert, Luca FRen
dc.contributor.authorSheu-Gruttadauria, Jessicaen
dc.contributor.authorMeredith, Lukeen
dc.contributor.authorLiu, Zhong-Yuen
dc.contributor.authorKwok, Chun Kiten
dc.contributor.authorQin, Cheng-Fengen
dc.contributor.authorMacRae, Ian Jen
dc.contributor.authorGoodfellow, Ianen
dc.contributor.authorMarioni, Johnen
dc.contributor.authorKudla, Grzegorzen
dc.contributor.authorMiska, Ericen
dc.date.accessioned2018-09-08T06:34:02Z
dc.date.available2018-09-08T06:34:02Z
dc.date.issued2018-10en
dc.identifier.issn1548-7091
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/279900
dc.description.abstractRNA structural flexibility underlies fundamental biological processes, but there are no methods to explore the multiple conformations adopted by RNAs in vivo. We developed Crosslinking Of Matched RNAs And Deep Sequencing (COMRADES) for in-depth RNA conformation capture, and a pipeline for retrieval of RNA structural ensembles. Using COMRADES, we determined the architecture of the Zika virus RNA genome inside cells, and revealed multiple site-specific interactions with human non-coding RNAs.
dc.description.sponsorshipThis work was supported by Cancer Research UK (C13474/A18583, C6946/A14492) and the Wellcome Trust (104640/Z/14/Z, 092096/Z/10/Z) to E.A.M. O.Z. was supported by the Human Frontier Science Program (HFSP, LT000558/2015), the European Molecular Biology Organization (EMBO, ALTF1622-2014), and the Blavatnik Family Foundation postdoctoral fellowship. G.K. and M.G. were supported by Wellcome Trust grant 207507 and UK Medical Research Council. A.T.L.L. and J.C.M. were supported by core funding from Cancer Research UK (award no. 17197 to JCM). J.C.M was also supported by core funding from EMBL. I.G. and L.W.M. were supported by the Wellcome Trust Senior Fellowship in Basic Biomedical Science to I.G. (207498/Z/17/Z). I.J.M., L.F.G. and J.S.-G. were supported by grants R01GM104475 and R01GM115649 from NIGMS. C.K.K was supported by City University of Hong Kong Projects 9610363 and 7200520, Croucher Foundation Project 9500030 and Hong Kong RGC Projects 9048103 and 9054020. C.-F.Q. was supported by the NSFC Excellent Young Scientist Fund 81522025 and the Newton Advanced Fellowship from the Academy of Medical Sciences, UK.
dc.format.mediumPrint-Electronicen
dc.languageengen
dc.publisherSpringer Nature
dc.subjectHumansen
dc.subjectRNA-Binding Proteinsen
dc.subjectRNA, Viralen
dc.subjectSequence Analysis, RNAen
dc.subjectNucleic Acid Conformationen
dc.subjectHigh-Throughput Nucleotide Sequencingen
dc.subjectTranscriptomeen
dc.subjectZika Virusen
dc.subjectZika Virus Infectionen
dc.titleCOMRADES determines in vivo RNA structures and interactions.en
dc.typeArticle
prism.endingPage788
prism.issueIdentifier10en
prism.publicationDate2018en
prism.publicationNameNature methodsen
prism.startingPage785
prism.volume15en
dc.identifier.doi10.17863/CAM.27268
dcterms.dateAccepted2018-07-20en
rioxxterms.versionofrecord10.1038/s41592-018-0121-0en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2018-10en
dc.contributor.orcidZiv, Omer [0000-0002-3044-5006]
dc.contributor.orcidGabryelska, Marta M [0000-0002-4433-282X]
dc.contributor.orcidLun, Aaron [0000-0002-3564-4813]
dc.contributor.orcidGebert, Luca FR [0000-0002-1830-119X]
dc.contributor.orcidMeredith, Luke [0000-0002-3802-8290]
dc.contributor.orcidLiu, Zhong-Yu [0000-0001-7385-5681]
dc.contributor.orcidKwok, Chun Kit [0000-0001-9175-8543]
dc.contributor.orcidQin, Cheng-Feng [0000-0002-0632-2807]
dc.contributor.orcidGoodfellow, Ian [0000-0002-9483-510X]
dc.contributor.orcidMarioni, John [0000-0001-9092-0852]
dc.contributor.orcidKudla, Grzegorz [0000-0002-7924-2744]
dc.contributor.orcidMiska, Eric [0000-0002-4450-576X]
dc.identifier.eissn1548-7105
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idCancer Research UK (18583)
pubs.funder-project-idWELLCOME TRUST (104640/Z/14/Z)
pubs.funder-project-idWellcome Trust (092096/Z/10/Z)
pubs.funder-project-idWellcome Trust (207498/Z/17/A)
pubs.funder-project-idWellcome Trust (207498/Z/17/Z)
pubs.funder-project-idCancer Research UK (A14492)
rioxxterms.freetoread.startdate2019-03-10


Files in this item

Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record