Show simple item record

dc.contributor.authorWhitty, Ciaraen
dc.contributor.authorWardale, R Johnen
dc.contributor.authorHenson, Francesen
dc.date.accessioned2018-09-10T22:14:39Z
dc.date.available2018-09-10T22:14:39Z
dc.date.issued2018-09en
dc.identifier.issn0006-291X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/279998
dc.description.abstractSclerostin is a clinically important protein with key functions in the musculoskeletal system playing a key role in bone formation and remodelling. Whilst a wide range range of mechanisms have been identified which regulate sclerostin expression, little is known about the degradation of the protein. The aim of this study was to identify enzymes capable of degrading sclerostin in peridontal ligament (PDL) fibroblasts cells in vitro and to investigate the functionality of these enzymes. We have demonstrated that cathepsin K cleaves sclerostin in vitro in PDL. We have shown that cathepsin K and sclerostin are co-localised in PDL cells and, using cathepsin K knockdown experiments, we have shown that cathepsin K actively controls sclerostin levels in these cells, through a lysosomal mechanism that is affected by hypoxia. These results are the first description of the degradative control of sclerostin in musculoskeletally derived cells in vitro and suggest that degradation of the protein may well play an important role in the control of bone formation and remodelling.
dc.description.sponsorshipInsall Foundation
dc.format.mediumPrint-Electronicen
dc.languageengen
dc.publisherElsevier
dc.subjectCell Lineen
dc.subjectFibroblastsen
dc.subjectPeriodontal Ligamenten
dc.subjectHumansen
dc.subjectAdaptor Proteins, Signal Transducingen
dc.subjectBone Morphogenetic Proteinsen
dc.subjectGenetic Markersen
dc.subjectCell Hypoxiaen
dc.subjectOsteogenesisen
dc.subjectCathepsin Ken
dc.subjectProteolysisen
dc.titleThe regulation of sclerostin by cathepsin K in periodontal ligament cells.en
dc.typeArticle
prism.endingPage555
prism.issueIdentifier2en
prism.publicationDate2018en
prism.publicationNameBiochemical and biophysical research communicationsen
prism.startingPage550
prism.volume503en
dc.identifier.doi10.17863/CAM.27364
dcterms.dateAccepted2018-05-23en
rioxxterms.versionofrecord10.1016/j.bbrc.2018.05.160en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2018-09en
dc.contributor.orcidHenson, Frances [0000-0002-3518-1492]
dc.identifier.eissn1090-2104
rioxxterms.typeJournal Article/Reviewen
rioxxterms.freetoread.startdate2019-09-30


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record