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dc.contributor.authorKaram Teixeira, F
dc.date.accessioned2018-09-10T22:15:01Z
dc.date.available2018-09-10T22:15:01Z
dc.identifier.issn1064-3745
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/280008
dc.description.abstractThe Drosophila melanogaster ovary is a powerful, genetically tractable system through which one can elucidate the principles underlying cellular function and organogenesis in vivo. In order to understand the intricate process of oogenesis at the subcellular level, microscopic analysis with the highest possible resolution is required. In this chapter, we describe the preparation of ovaries for ultrastructural analysis using transmission electron microscopy and focused ion beam scanning electron microscopy. We discuss and provide protocols for chemical fixation of Drosophila ovaries that facilitate optimal imaging with particular attention paid to preserving and resolving mitochondrial membrane morphology and structure.
dc.publisherSpringer Nature
dc.titleUltrastructural Analysis of Drosophila Ovaries by Electron Microscopy
dc.typeArticle
prism.publicationNameMethods in molecular biology (Clifton, N.J.)
dc.identifier.doi10.17863/CAM.27373
rioxxterms.versionofrecord10.1007/978-1-4939-2851-4_11
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
dc.contributor.orcidKaram Teixeira, Felipe [0000-0001-7651-1657]
rioxxterms.typeJournal Article/Review
rioxxterms.freetoread.startdate2019-09-09


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