NMR spectroscopy of native and in vitro tissues implicates polyADP ribose in biomineralization.
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Authors
Chow, W Ying
Muller, Karin H
Reid, David G
Skepper, Jeremy N
Wong, Wai Ching
Brooks, Roger A
Green, Maggie
Bihan, Dominique
Farndale, Richard W
Slatter, David A
Shanahan, Catherine M
Duer, Melinda J
Publication Date
2014-05-16Journal Title
Science
ISSN
0036-8075
Publisher
American Association for the Advancement of Science (AAAS)
Volume
344
Issue
6185
Pages
742-746
Language
eng
Type
Article
Physical Medium
Print
Metadata
Show full item recordCitation
Chow, W. Y., Rajan, R., Muller, K. H., Reid, D. G., Skepper, J. N., Wong, W. C., Brooks, R. A., et al. (2014). NMR spectroscopy of native and in vitro tissues implicates polyADP ribose in biomineralization.. Science, 344 (6185), 742-746. https://doi.org/10.1126/science.1248167
Abstract
Nuclear magnetic resonance (NMR) spectroscopy is useful to determine molecular structure in tissues grown in vitro only if their fidelity, relative to native tissue, can be established. Here, we use multidimensional NMR spectra of animal and in vitro model tissues as fingerprints of their respective molecular structures, allowing us to compare the intact tissues at atomic length scales. To obtain spectra from animal tissues, we developed a heavy mouse enriched by about 20% in the NMR-active isotopes carbon-13 and nitrogen-15. The resulting spectra allowed us to refine an in vitro model of developing bone and to probe its detailed structure. The identification of an unexpected molecule, poly(adenosine diphosphate ribose), that may be implicated in calcification of the bone matrix, illustrates the analytical power of this approach.
Keywords
Animals, Bone Development, Calcification, Physiologic, Carbon Isotopes, Extracellular Matrix, Growth Plate, Mice, Models, Biological, Nitrogen Isotopes, Nuclear Magnetic Resonance, Biomolecular, Poly Adenosine Diphosphate Ribose, Sheep
Sponsorship
Medical Research Council (G0500707)
British Heart Foundation (None)
British Heart Foundation (None)
Biotechnology and Biological Sciences Research Council (BB/G021392/1)
British Heart Foundation (None)
Identifiers
External DOI: https://doi.org/10.1126/science.1248167
This record's URL: https://www.repository.cam.ac.uk/handle/1810/280114
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