Assessing Ubiquitylation of Individual Proteins Using Xenopus Extract Systems.
Cold Spring Harb Protoc
Cold Spring Harbor Laboratory
MetadataShow full item record
McDowell, G. S., & Philpott, A. (2019). Assessing Ubiquitylation of Individual Proteins Using Xenopus Extract Systems.. Cold Spring Harb Protoc, 2019 (6) https://doi.org/10.1101/pdb.prot104513
Xenopus extract systems have been used to study ubiquitylation of proteins, and to uncover some of the fundamental processes of the ubiquitylation pathway itself. They provide a simple, quick, and robust method for studying ubiquitylation. In this protocol, methods are provided for studying protein ubiquitylation using Xenopus egg or embryo extracts and in vitro radiolabeled proteins. These methods also enable examination of whether proteins undergo noncanonical ubiquitylation, through modification of the protein by covalent linkage to ubiquitin through residues other than lysine, such as cysteine, serine, and threonine.
Animals, Biochemistry, Biological Assay, Cell Extracts, Protein Biosynthesis, Radiopharmaceuticals, Transcription, Genetic, Ubiquitination, Xenopus Proteins, Xenopus laevis
Medical Research Council (MC_PC_12009)
Medical Research Council (MR/K018329/1)
Medical Research Council (MR/L021129/1)
External DOI: https://doi.org/10.1101/pdb.prot104513
This record's URL: https://www.repository.cam.ac.uk/handle/1810/280344
Recommended or similar items
The current recommendation prototype on the Apollo Repository will be turned off on 03 February 2023. Although the pilot has been fruitful for both parties, the service provider IKVA is focusing on horizon scanning products and so the recommender service can no longer be supported. We recognise the importance of recommender services in supporting research discovery and are evaluating offerings from other service providers. If you would like to offer feedback on this decision please contact us on: email@example.com