Show simple item record

dc.contributor.authorMcDowell, Gary S
dc.contributor.authorPhilpott, Anna
dc.date.accessioned2018-09-19T11:39:36Z
dc.date.available2018-09-19T11:39:36Z
dc.date.issued2019-06-03
dc.identifier.issn1940-3402
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/280344
dc.description.abstractXenopus extract systems have been used to study ubiquitylation of proteins, and to uncover some of the fundamental processes of the ubiquitylation pathway itself. They provide a simple, quick, and robust method for studying ubiquitylation. In this protocol, methods are provided for studying protein ubiquitylation using Xenopus egg or embryo extracts and in vitro radiolabeled proteins. These methods also enable examination of whether proteins undergo noncanonical ubiquitylation, through modification of the protein by covalent linkage to ubiquitin through residues other than lysine, such as cysteine, serine, and threonine.
dc.format.mediumElectronic
dc.languageeng
dc.publisherCold Spring Harbor Laboratory
dc.subjectAnimals
dc.subjectXenopus laevis
dc.subjectXenopus Proteins
dc.subjectCell Extracts
dc.subjectRadiopharmaceuticals
dc.subjectBiological Assay
dc.subjectBiochemistry
dc.subjectProtein Biosynthesis
dc.subjectTranscription, Genetic
dc.subjectUbiquitination
dc.titleAssessing Ubiquitylation of Individual Proteins Using Xenopus Extract Systems.
dc.typeArticle
prism.issueIdentifier6
prism.publicationDate2019
prism.publicationNameCold Spring Harb Protoc
prism.volume2019
dc.identifier.doi10.17863/CAM.27715
dcterms.dateAccepted2018-04-19
rioxxterms.versionofrecord10.1101/pdb.prot104513
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2019-06-03
dc.contributor.orcidMcDowell, Gary S [0000-0002-9470-3799]
dc.contributor.orcidPhilpott, Anna [0000-0003-3789-2463]
dc.identifier.eissn1559-6095
rioxxterms.typeJournal Article/Review
pubs.funder-project-idMedical Research Council (MC_PC_12009)
pubs.funder-project-idMedical Research Council (MR/K018329/1)
pubs.funder-project-idMedical Research Council (MR/L021129/1)
cam.issuedOnline2018-05-16


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record