Calculating the Degradation Rate of Individual Proteins Using Xenopus Extract Systems.
Accepted version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Philpott, Anna
Abstract
The Xenopus extract system has been used extensively as a simple, quick, and robust method for assessing the stability of proteins against proteasomal degradation. In this protocol, methods are provided for assessing the half-life of in vitro translated radiolabeled proteins using Xenopus egg or embryo extracts.
Description
Keywords
Animals, Biochemistry, Biological Assay, Cell Extracts, Protein Biosynthesis, Proteolysis, Radiopharmaceuticals, Transcription, Genetic, Xenopus Proteins, Xenopus laevis
Journal Title
Cold Spring Harb Protoc
Conference Name
Journal ISSN
1940-3402
1559-6095
1559-6095
Volume Title
2019
Publisher
Cold Spring Harbor Laboratory
Publisher DOI
Sponsorship
Medical Research Council (MC_PC_12009)
Medical Research Council (MR/K018329/1)
Medical Research Council (MR/L021129/1)
Medical Research Council (MR/K018329/1)
Medical Research Council (MR/L021129/1)