Calculating the Degradation Rate of Individual Proteins Using Xenopus Extract Systems.

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Authors
  McDowell, Gary S
Philpott, Anna
Publication Date
2019-06-03
Journal Title
Cold Spring Harb Protoc
ISSN
1940-3402
Publisher
Cold Spring Harbor Laboratory
Volume
2019
Issue
6
Language
eng
Type
Article
This Version
AM
Physical Medium
Electronic
Metadata
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Citation
McDowell, G. S., & Philpott, A. (2019). Calculating the Degradation Rate of Individual Proteins Using Xenopus Extract Systems.. Cold Spring Harb Protoc, 2019 (6) https://doi.org/10.1101/pdb.prot103481
Abstract
The Xenopus extract system has been used extensively as a simple, quick, and robust method for assessing the stability of proteins against proteasomal degradation. In this protocol, methods are provided for assessing the half-life of in vitro translated radiolabeled proteins using Xenopus egg or embryo extracts.
Keywords
Animals, Xenopus laevis, Xenopus Proteins, Cell Extracts, Radiopharmaceuticals, Biological Assay, Biochemistry, Protein Biosynthesis, Transcription, Genetic, Proteolysis
Sponsorship
Medical Research Council (MC_PC_12009)
Medical Research Council (MR/K018329/1)
Medical Research Council (MR/L021129/1)
Identifiers
External DOI: https://doi.org/10.1101/pdb.prot103481
This record's URL: https://www.repository.cam.ac.uk/handle/1810/280349
Rights
Licence:
http://www.rioxx.net/licenses/all-rights-reserved
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