Calculating the Degradation Rate of Individual Proteins Using Xenopus Extract Systems.

McDowell, Gary S; Philpott, Anna

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Authors

McDowell, Gary S

Philpott, Anna

Publication Date

2019-06-03

Journal Title

Cold Spring Harb Protoc

ISSN

1940-3402

Publisher

Cold Spring Harbor Laboratory

Volume

2019

Issue

Language

eng

Type

Article

This Version

Physical Medium

Electronic

Metadata

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Citation

McDowell, G. S., & Philpott, A. (2019). Calculating the Degradation Rate of Individual Proteins Using Xenopus Extract Systems.. Cold Spring Harb Protoc, 2019 (6) https://doi.org/10.1101/pdb.prot103481

Abstract

The Xenopus extract system has been used extensively as a simple, quick, and robust method for assessing the stability of proteins against proteasomal degradation. In this protocol, methods are provided for assessing the half-life of in vitro translated radiolabeled proteins using Xenopus egg or embryo extracts.

Keywords

Animals, Xenopus laevis, Xenopus Proteins, Cell Extracts, Radiopharmaceuticals, Biological Assay, Biochemistry, Protein Biosynthesis, Transcription, Genetic, Proteolysis

Sponsorship

Medical Research Council (MC_PC_12009)

Medical Research Council (MR/K018329/1)

Medical Research Council (MR/L021129/1)

Identifiers

External DOI: https://doi.org/10.1101/pdb.prot103481

This record's URL: https://www.repository.cam.ac.uk/handle/1810/280349

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Licence:

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