dc.contributor.author Savin, TH dc.contributor.author Bogdan, Michał dc.date.accessioned 2018-09-21T14:44:29Z dc.date.available 2018-09-21T14:44:29Z dc.date.issued 2018-07-03 dc.identifier.issn 1542-0086 dc.identifier.uri https://www.repository.cam.ac.uk/handle/1810/280607 dc.description.abstract Tracking Brownian particles is often employed to map the energy landscape they explore. Such measurements have been exploited to study many biological processes and interactions in soft materials. Yet, video tracking is irremediably contaminated by localization errors originating from two imaging artifacts: the static'' errors come from signal noise, and the dynamic'' errors arise from the motion blur due to finite frame acquisition time. We show that these errors result in systematic and non-trivial biases in the measured energy landscapes. We derive a relationship between the true and the measured potential that elucidates, among other aberrations, the presence of false double-well minima in the apparent potentials reported in recent studies. We further assess several canonical trapping and pair-interaction potentials, by using our analytically derived results and Brownian dynamics simulations. In particular, we show that the apparent spring stiffness of harmonic potentials (such as optical traps) is increased by dynamic errors, but decreased by static errors. Our formula allows for the development of efficient corrections schemes, and we also present in this paper a provisional method for reconstructing true potentials from the measured ones. dc.language eng dc.publisher Elsevier dc.rights Attribution-NonCommercial-NoDerivatives 4.0 International dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ dc.title Errors in energy landscapes measured with particle tracking dc.type Article prism.endingPage 149 prism.issueIdentifier 1 prism.publicationName Biophysical Journal prism.startingPage 139 prism.volume 115 dc.identifier.doi 10.17863/CAM.27974 dcterms.dateAccepted 2018-05-01 rioxxterms.versionofrecord 10.1016/j.bpj.2018.05.035 rioxxterms.version AM rioxxterms.licenseref.uri http://www.rioxx.net/licenses/all-rights-reserved rioxxterms.licenseref.startdate 2018-05-01 dc.contributor.orcid Savin, Thierry [0000-0002-3956-7077] dc.identifier.eissn 1542-0086 rioxxterms.type Journal Article/Review cam.issuedOnline 2018-07-03 rioxxterms.freetoread.startdate 2019-07-03
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