Stiffening by Osmotic Swelling Constraint in Cartilage-Like Cell Culture Scaffolds.
Offeddu, Giovanni S
Tanase, Constantin E
Oyen, Michelle L
Cameron, Ruth E
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Offeddu, G. S., Tanase, C. E., Toumpaniari, S., Oyen, M. L., & Cameron, R. E. (2018). Stiffening by Osmotic Swelling Constraint in Cartilage-Like Cell Culture Scaffolds.. Macromol Biosci, 18 (11), e1800247. https://doi.org/10.1002/mabi.201800247
Cartilage wounds result in chronic pain and degradation of the quality of life for millions of people. A synthetic cellular scaffold able to heal the damage by substituting the natural tissue is of great potential value. Here, it is shown for the first time that the unique interplay between the molecular components of cartilage can be reproduced in composite materials made of a polyelectrolyte hydrogel embedding a collagen scaffold. These composites possess a mechanical response determined by osmotic and electrostatic effects, comparable to articular cartilage in terms of elastic modulus, time-dependent response, and permeability to interstitial fluid flow. Made entirely from biocompatible materials, the cartilage-like composite materials developed permit 3D culture of chondrocyte-like cells through their microporosity. The biomimetic materials presented here constitute an entirely new class of osmotically stiffened composites, which may find use outside of biomedical applications.
biomimetic cell microenvironments, collagen, composite materials, mechanical properties, polyelectrolytes, Biocompatible Materials, Biomimetic Materials, Cartilage, Cell Culture Techniques, Cell Line, Tumor, Collagen, Elastic Modulus, Humans, Hydrogels, Osmotic Pressure, Static Electricity, Tissue Scaffolds
This work was supported by the European Research Council [ERC Advanced Grant 320598 3D-E], and the Engineering and Physical Sciences Research Council [grant EPSRC EP/G037221/1].
European Research Council (320598)
Engineering and Physical Sciences Research Council (EP/G037221/1)
External DOI: https://doi.org/10.1002/mabi.201800247
This record's URL: https://www.repository.cam.ac.uk/handle/1810/280614
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