Abnormalities of age-related T cell senescence in Parkinson's disease.
Journal of neuroinflammation
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Williams-Gray, C., Wijeyekoon, R., Scott, K., Hayat, S., Barker, R., & Jones, J. (2018). Abnormalities of age-related T cell senescence in Parkinson's disease.. Journal of neuroinflammation, 15 (1), 166. https://doi.org/10.1186/s12974-018-1206-5
Background: A wealth of evidence implicates both central and peripheral immune changes as contributing to the pathogenesis of Parkinson’s disease (PD). It is critical to better understand this aspect of PD given that it is a tractable target for disease modifying therapy. Age-related changes are known to occur in the immune system (immunosenescence) and might be of particular relevance in PD given that its prevalence rises with increasing age. We therefore sought to investigate this with respect to T cell replicative senescence, a key immune component of human ageing. Methods: Peripheral blood mononuclear cells were extracted from blood samples from 41 patients with mild PD (Hoehn and Yahr stage 1-2, mean(SD) disease duration 4.3(1.2) years) and 41 age and gender matched controls. Immunophenotyping was performed with flow cytometry using markers of T-lymphocyte activation and senescence (CD3, CD4, CD8, HLA-DR, CD38, CD28, CCR7, CD45RA, CD57, CD31). Cytomegalovirus (CMV) serology was measured given its proposed relevance in driving T cell senescence. Results: Markers of replicative senescence in the CD8+ population were strikingly reduced in PD cases versus controls (reduced CD57 expression (p=0.005), reduced percentage of ‘late differentiated’ CD57loCD28hi cells (p=0.007) and ‘TEMRA’ cells (p=0.042)), whilst expression of activation markers (CD28) was increased (p=0.005). This was not driven by differences in CMV seropositivity. No significant changes were observed in the CD4 population. Conclusions: This study demonstrates for the first time that the peripheral immune profile in PD is distinctly atypical for an older population, with a lack of the CD8+ T cell replicative senescence which characterises normal ageing. This suggests that ‘abnormal’ immune ageing may contribute to the development of PD, and markers
Leukocytes, Mononuclear, T-Lymphocytes, Humans, Cytomegalovirus, Parkinson Disease, Immunoglobulin G, Antigens, CD, Flow Cytometry, Case-Control Studies, Immunophenotyping, Serology, Aging, Aged, Middle Aged, Female, Male, Immunosenescence, Cellular Senescence
Grant funding from the Academy of Medical Sciences UK, the Rosetrees Trust, the Stevenage Biosciences Catalyst and Addenbrooke’s Charitable Trust contributed to this work. The research was also supported by the NIHR Cambridge Biomedical Research Centre (Cambridge University Hospitals NHS Trust/University of Cambridge). C H Williams-Gray is supported by a fellowship from the Medical Research Council. R S Wijeyekoon was supported by a fellowship from Addenbrooke’s Charitable Trust. K M Scott and J L Jones are supported by fellowships from the Wellcome Trust. R A Barker is supported by the Wellcome-MRC Cambridge Stem Cell Institute.
Wellcome Trust (105924/Z/14/A)
Academy of Medical Sciences (unknown)
Addenbrooke's Charitable Trust (ACT) (PF15/CWG)
Stevenage Bioscience Catalyst (unknown)
External DOI: https://doi.org/10.1186/s12974-018-1206-5
This record's URL: https://www.repository.cam.ac.uk/handle/1810/280744
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/