A quantitative mass spectrometry-based approach to monitor the dynamics of endogenous chromatin-associated protein complexes.
dc.contributor.author | Papachristou, Evangelia K | |
dc.contributor.author | Kishore, Kamal | |
dc.contributor.author | Holding, Andrew N | |
dc.contributor.author | Harvey, Kate | |
dc.contributor.author | Roumeliotis, Theodoros I | |
dc.contributor.author | Chilamakuri, Chandra Sekhar Reddy | |
dc.contributor.author | Omarjee, Soleilmane | |
dc.contributor.author | Chia, Kee Ming | |
dc.contributor.author | Swarbrick, Alex | |
dc.contributor.author | Lim, Elgene | |
dc.contributor.author | Markowetz, Florian | |
dc.contributor.author | Eldridge, Matthew | |
dc.contributor.author | Siersbaek, Rasmus | |
dc.contributor.author | D'Santos, Clive S | |
dc.contributor.author | Carroll, Jason S | |
dc.date.accessioned | 2018-09-26T13:00:14Z | |
dc.date.available | 2018-09-26T13:00:14Z | |
dc.date.issued | 2018-06-13 | |
dc.identifier.issn | 2041-1723 | |
dc.identifier.uri | https://www.repository.cam.ac.uk/handle/1810/280762 | |
dc.description.abstract | Understanding the dynamics of endogenous protein-protein interactions in complex networks is pivotal in deciphering disease mechanisms. To enable the in-depth analysis of protein interactions in chromatin-associated protein complexes, we have previously developed a method termed RIME (Rapid Immunoprecipitation Mass spectrometry of Endogenous proteins). Here, we present a quantitative multiplexed method (qPLEX-RIME), which integrates RIME with isobaric labelling and tribrid mass spectrometry for the study of protein interactome dynamics in a quantitative fashion with increased sensitivity. Using the qPLEX-RIME method, we delineate the temporal changes of the Estrogen Receptor alpha (ERα) interactome in breast cancer cells treated with 4-hydroxytamoxifen. Furthermore, we identify endogenous ERα-associated proteins in human Patient-Derived Xenograft tumours and in primary human breast cancer clinical tissue. Our results demonstrate that the combination of RIME with isobaric labelling offers a powerful tool for the in-depth and quantitative characterisation of protein interactome dynamics, which is applicable to clinical samples. | |
dc.language | eng | |
dc.publisher | Springer Nature | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Breast cancer | |
dc.subject | Immunoprecipitation | |
dc.subject | Mass spectrometry | |
dc.subject | Protein–protein interaction networks | |
dc.title | A quantitative mass spectrometry-based approach to monitor the dynamics of endogenous chromatin-associated protein complexes. | |
dc.type | Article | |
prism.endingPage | 2311 | |
prism.issueIdentifier | 1 | |
prism.number | 2311 | |
prism.publicationDate | 2018 | |
prism.publicationName | Nature Communications | |
prism.startingPage | 2311 | |
prism.volume | 9 | |
dc.identifier.doi | 10.17863/CAM.28126 | |
dcterms.dateAccepted | 2018-05-03 | |
rioxxterms.versionofrecord | 10.1038/s41467-018-04619-5 | |
rioxxterms.version | VoR | |
rioxxterms.licenseref.uri | http://creativecommons.org/licenses/by/4.0/ | |
rioxxterms.licenseref.startdate | 2018-06-13 | |
dc.contributor.orcid | Papachristou, Eva [0000-0002-5835-2055] | |
dc.contributor.orcid | Kishore, Kamal [0000-0002-4650-8745] | |
dc.contributor.orcid | Holding, Andrew [0000-0002-8459-7048] | |
dc.contributor.orcid | Markowetz, Florian [0000-0002-2784-5308] | |
dc.contributor.orcid | Eldridge, Matthew [0000-0002-5799-8911] | |
dc.contributor.orcid | Carroll, Jason [0000-0003-3643-0080] | |
dc.identifier.eissn | 2041-1723 | |
dc.publisher.url | https://www.nature.com/articles/s41467-018-04619-5#article-info | |
rioxxterms.type | Journal Article/Review | |
pubs.funder-project-id | Wellcome Trust (108467/Z/15/Z) | |
pubs.funder-project-id | Cancer Research UK (C14303/A17197) | |
pubs.funder-project-id | Breast Cancer Campaign (2012NovemberPR042) | |
pubs.funder-project-id | European Research Council (646876) | |
pubs.funder-project-id | Cancer Research UK (A19274) | |
cam.issuedOnline | 2018-06-13 |
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