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dc.contributor.authorZeiser, Eva
dc.contributor.authorFrøkjær-Jensen, Christian
dc.contributor.authorJorgensen, Erik
dc.contributor.authorAhringer, Julie
dc.date.accessioned2018-09-27T14:09:54Z
dc.date.available2018-09-27T14:09:54Z
dc.date.issued2011
dc.identifier.issn1932-6203
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/282789
dc.description.abstractHere we describe a toolkit for the production of fluorescently tagged proteins in the C. elegans germline and early embryo using Mos1-mediated single copy insertion (MosSCI) transformation. We have generated promoter and 3'UTR fusions to sequences of different fluorescent proteins yielding constructs for germline expression that are compatible with MosSCI MultiSite Gateway vectors. These vectors allow tagged transgene constructs to be inserted as single copies into known sites in the C. elegans genome using MosSCI. We also show that two C. elegans heat shock promoters (Phsp-16.2 and Phsp-16.41) can be used to induce transgene expression in the germline when inserted via MosSCI transformation. This flexible set of new vectors, available to the research community in a plasmid repository, should facilitate research focused on the C. elegans germline and early embryo.
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherPublic Library of Science (PLoS)
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectGerm Cells
dc.subjectAnimals
dc.subjectAnimals, Genetically Modified
dc.subjectCaenorhabditis elegans
dc.subjectCaenorhabditis elegans Proteins
dc.subject3' Untranslated Regions
dc.subjectGene Expression Regulation
dc.subjectHeat-Shock Response
dc.subjectTransgenes
dc.subjectGenetic Vectors
dc.subjectPlasmids
dc.subjectPromoter Regions, Genetic
dc.titleMosSCI and gateway compatible plasmid toolkit for constitutive and inducible expression of transgenes in the C. elegans germline.
dc.typeArticle
prism.issueIdentifier5
prism.publicationDate2011
prism.publicationNamePLoS One
prism.startingPagee20082
prism.volume6
dc.identifier.doi10.17863/CAM.30153
dcterms.dateAccepted2011-04-18
rioxxterms.versionofrecord10.1371/journal.pone.0020082
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2011-01
dc.contributor.orcidAhringer, Julie [0000-0002-7074-4051]
dc.identifier.eissn1932-6203
rioxxterms.typeJournal Article/Review
pubs.funder-project-idWellcome Trust (054523/Z/98/C)
cam.issuedOnline2011-05-26


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International