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dc.contributor.authorHume, Robert D
dc.contributor.authorPensa, Sara
dc.contributor.authorBrown, Elizabeth J
dc.contributor.authorKreuzaler, Peter A
dc.contributor.authorHitchcock, Jessica
dc.contributor.authorHusmann, Anke
dc.contributor.authorCampbell, Jonathan J
dc.contributor.authorLloyd-Thomas, Annabel O
dc.contributor.authorCameron, Ruth
dc.contributor.authorWatson, Christine
dc.date.accessioned2018-09-27T14:11:07Z
dc.date.available2018-09-27T14:11:07Z
dc.date.issued2018-08-23
dc.identifier.issn2045-2322
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/282813
dc.description.abstractBreast cancers are highly heterogeneous and their metastatic potential and response to therapeutic drugs is difficult to predict. A tool that could accurately gauge tumour invasiveness and drug response would provide a valuable addition to the oncologist's arsenal. We have developed a 3-dimensional (3D) culture model that recapitulates the stromal environment of breast cancers by generating anisotropic (directional) collagen scaffolds seeded with adipocytes and culturing tumour fragments therein. Analysis of tumour cell invasion in the presence of various therapeutic drugs, by immunofluorescence microscopy coupled with an optical clearing technique, demonstrated the utility of this approach in determining both the rate and capacity of tumour cells to migrate through the stroma while shedding light also on the mode of migration. Furthermore, the response of different murine mammary tumour types to chemotherapeutic drugs could be readily quantified.
dc.format.mediumElectronic
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject3T3-L1 Cells
dc.subjectAdipocytes
dc.subjectAnimals
dc.subjectMice, Inbred BALB C
dc.subjectHumans
dc.subjectMice
dc.subjectCollagen
dc.subjectMicroscopy, Confocal
dc.subjectMicroscopy, Fluorescence
dc.subjectTissue Engineering
dc.subjectImmunohistochemistry
dc.subjectCell Movement
dc.subjectFemale
dc.subjectTissue Scaffolds
dc.titleTumour cell invasiveness and response to chemotherapeutics in adipocyte invested 3D engineered anisotropic collagen scaffolds.
dc.typeArticle
prism.issueIdentifier1
prism.publicationDate2018
prism.publicationNameSci Rep
prism.startingPage12658
prism.volume8
dc.identifier.doi10.17863/CAM.30177
dcterms.dateAccepted2018-06-05
rioxxterms.versionofrecord10.1038/s41598-018-30107-3
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-08-23
dc.contributor.orcidKreuzaler, Peter A [0000-0001-8684-9424]
dc.contributor.orcidHusmann, Anke [0000-0001-5326-3785]
dc.contributor.orcidCameron, Ruth [0000-0003-1573-4923]
dc.contributor.orcidWatson, Christine [0000-0002-8548-5902]
dc.identifier.eissn2045-2322
rioxxterms.typeJournal Article/Review
pubs.funder-project-idMedical Research Council (MR/K011014/1)
pubs.funder-project-idEuropean Research Council (320598)
pubs.funder-project-idMedical Research Council (MR/N022963/1)
pubs.funder-project-idNational Centre for the Replacement Refinement and Reduction of Animals in Research (NC/K001655/1)
cam.issuedOnline2018-08-23


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International