Developmental characterization of the microRNA-specific C. elegans Argonautes alg-1 and alg-2.
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Authors
Vasquez-Rifo, Alejandro
Jannot, Guillaume
Armisen, Javier
Labouesse, Michel
Bukhari, Syed Irfan Ahmad
Rondeau, Evelyne L
Miska, Eric A
Simard, Martin J
Publication Date
2012Journal Title
PLoS One
ISSN
1932-6203
Publisher
Public Library of Science (PLoS)
Volume
7
Issue
3
Pages
e33750
Language
eng
Type
Article
Physical Medium
Print-Electronic
Metadata
Show full item recordCitation
Vasquez-Rifo, A., Jannot, G., Armisen, J., Labouesse, M., Bukhari, S. I. A., Rondeau, E. L., Miska, E. A., & et al. (2012). Developmental characterization of the microRNA-specific C. elegans Argonautes alg-1 and alg-2.. PLoS One, 7 (3), e33750. https://doi.org/10.1371/journal.pone.0033750
Abstract
The genes alg-1 and alg-2 (referred to as "alg-1/2") encode the Argonaute proteins affiliated to the microRNA (miRNA) pathway in C. elegans. Bound to miRNAs they form the effector complex that effects post-transcriptional gene silencing. In order to define biological features important to understand the mode of action of these Argonautes, we characterize aspects of these genes during development. We establish that alg-1/2 display an overlapping spatio-temporal expression profile and shared association to a miRNAs set, but with gene-specific predominant expression in various cells and increased relative association to defined miRNAs. Congruent with their spatio-temporal coincidence and regardless of alg-1/2 drastic post-embryonic differences, only loss of both genes leads to embryonic lethality. Embryos without zygotic alg-1/2 predominantly arrest during the morphogenetic process of elongation with defects in the epidermal-muscle attachment structures. Altogether our results highlight similarities and specificities of the alg-1/2 likely to be explained at different cellular and molecular levels.
Keywords
Muscle, Smooth, Epidermis, Embryo, Nonmammalian, Animals, Animals, Genetically Modified, Caenorhabditis elegans, RNA-Binding Proteins, Caenorhabditis elegans Proteins, MicroRNAs, Gene Expression Regulation, Developmental, Amino Acid Sequence, Sequence Homology, Amino Acid, Molecular Sequence Data, Epidermal Cells
Sponsorship
Wellcome Trust (092096/Z/10/Z)
Identifiers
External DOI: https://doi.org/10.1371/journal.pone.0033750
This record's URL: https://www.repository.cam.ac.uk/handle/1810/282827
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