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dc.contributor.authorLager, Susanne
dc.contributor.authorde Goffau, Marcus
dc.contributor.authorSovio, Ulla
dc.contributor.authorPeacock, Sharon
dc.contributor.authorParkhill, Julian
dc.contributor.authorCharnock-Jones, Stephen
dc.contributor.authorSmith, Gordon
dc.date.accessioned2018-10-03T04:44:57Z
dc.date.available2018-10-03T04:44:57Z
dc.date.issued2018-09-01
dc.identifier.issn2049-2618
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/283076
dc.description.abstractBACKGROUND: Fetal growth restriction, pre-eclampsia, and pre-term birth are major adverse pregnancy outcomes. These complications are considerable contributors to fetal/maternal morbidity and mortality worldwide. A significant proportion of these cases are thought to be due to dysfunction of the placenta. However, the underlying mechanisms of placental dysfunction are unclear. The aim of the present study was to investigate whether adverse pregnancy outcomes are associated with evidence of placental eukaryotic infection. RESULTS: We modified the 18S Illumina Amplicon Protocol of the Earth Microbiome Project and made it capable of detecting just a single spiked-in genome copy of Plasmodium falciparum, Saccharomyces cerevisiae, or Toxoplasma gondii among more than 70,000 human cells. Using this method, we were unable to detect eukaryotic pathogens in placental biopsies in instances of adverse pregnancy outcome (n = 199) or in healthy controls (n = 99). CONCLUSIONS: Eukaryotic infection of the placenta is not an underlying cause of the aforementioned pregnancy complications. Possible clinical applications for this non-targeted, yet extremely sensitive, eukaryotic screening method are manifest.
dc.description.sponsorshipNational Institute for Health Research (NIHR) Cambridge Biomedical Research Centre (Women’s Health theme) Wellcome Trust Sanger Institute
dc.format.mediumElectronic
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectPlacenta
dc.subjectHumans
dc.subjectToxoplasma
dc.subjectPlasmodium falciparum
dc.subjectSaccharomyces cerevisiae
dc.subjectPregnancy Complications
dc.subjectPregnancy Outcome
dc.subjectPregnancy
dc.subjectAdult
dc.subjectFemale
dc.subjectYoung Adult
dc.subjectEukaryota
dc.titleDetecting eukaryotic microbiota with single-cell sensitivity in human tissue.
dc.typeArticle
prism.issueIdentifier1
prism.publicationDate2018
prism.publicationNameMicrobiome
prism.startingPage151
prism.volume6
dc.identifier.doi10.17863/CAM.30438
dcterms.dateAccepted2018-08-09
rioxxterms.versionofrecord10.1186/s40168-018-0529-x
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-09
dc.contributor.orcidLager, Susanne [0000-0003-3556-065X]
dc.contributor.orcidde Goffau, Marcus [0000-0001-7032-1852]
dc.contributor.orcidSovio, Ulla [0000-0002-0799-1105]
dc.contributor.orcidPeacock, Sharon [0000-0002-1718-2782]
dc.contributor.orcidParkhill, Julian [0000-0002-7069-5958]
dc.contributor.orcidCharnock-Jones, Stephen [0000-0002-2936-4890]
dc.contributor.orcidSmith, Gordon [0000-0003-2124-0997]
dc.identifier.eissn2049-2618
rioxxterms.typeJournal Article/Review
pubs.funder-project-idMedical Research Council (MR/K021133/1)
pubs.funder-project-idCambridge University Hospitals NHS Foundation Trust (CUH) (146281)
pubs.funder-project-idMedical Research Council (G1100221)
cam.issuedOnline2018-09-01


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International