DDX3X RNA helicase affects breast cancer cell cycle progression by regulating expression of KLF4.
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Cannizzaro, E., Bannister, A., Han, N., Alendar, A., & Kouzarides, T. (2018). DDX3X RNA helicase affects breast cancer cell cycle progression by regulating expression of KLF4.. FEBS Letters, 592 (13), 2308-2322. https://doi.org/10.1002/1873-3468.13106
DDX3X is a multifunctional RNA helicase with documented roles in different cancer types. Here, we demonstrate that DDX3X plays an oncogenic role in breast cancer cells by modulating the cell cycle. Depletion of DDX3X in MCF7 cells slows cell proliferation by inducing a G1 phase arrest. Notably, DDX3X inhibits expression of Kruppel-like factor 4 (KLF4), a transcription factor and cell cycle repressor. Moreover, DDX3X directly interacts with KLF4 mRNA and regulates its splicing. We show that DDX3X-mediated repression of KLF4 promotes expression of S-phase inducing genes in MCF7 breast cancer cells. These findings provide evidence for a novel function of DDX3X in regulating expression and downstream functions of KLF4, a master negative regulator of the cell cycle.
DDX3X, KLF4, RNA helicase, breast cancer, cell cycle
Cancer Research UK (10827)
Cancer Research UK (17001)
Wellcome Trust (092096/Z/10/Z)
Cancer Research UK (A14492)
Embargo Lift Date
External DOI: https://doi.org/10.1002/1873-3468.13106
This record's URL: https://www.repository.cam.ac.uk/handle/1810/283185
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/