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dc.contributor.authorGrant, Rhysen
dc.contributor.authorAbdelbaki, Ahmeden
dc.contributor.authorBertoldi, Alessiaen
dc.contributor.authorGavilan, Maria Pen
dc.contributor.authorMansfeld, Jörgen
dc.contributor.authorGlover, Daviden
dc.contributor.authorLindon, Cathen
dc.date.accessioned2018-10-05T13:02:52Z
dc.date.available2018-10-05T13:02:52Z
dc.date.issued2018-06en
dc.identifier.issn2046-2441
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/283208
dc.description.abstractAurora A kinase (AURKA) is a major regulator of mitosis and an important driver of cancer progression. The roles of AURKA outside of mitosis, and how these might contribute to cancer progression, are not well understood. Here, we show that a fraction of cytoplasmic AURKA is associated with mitochondria, co-fractionating in cell extracts and interacting with mitochondrial proteins by reciprocal co-immunoprecipitation. We have also found that the dynamics of the mitochondrial network are sensitive to AURKA inhibition, depletion or overexpression. This can account for the different mitochondrial morphologies observed in RPE-1 and U2OS cell lines, which show very different levels of expression of AURKA. We identify the mitochondrial fraction of AURKA as influencing mitochondrial morphology, because an N-terminally truncated version of the kinase that does not localize to mitochondria does not affect the mitochondrial network. We identify a cryptic mitochondrial targeting sequence in the AURKA N-terminus and discuss how alternative conformations of the protein may influence its cytoplasmic fate.
dc.description.sponsorshipMRC CRUK
dc.format.mediumPrinten
dc.languageengen
dc.publisherThe Royal Society
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectCell Lineen
dc.subjectCytoplasmen
dc.subjectMitochondriaen
dc.subjectHumansen
dc.subjectMitochondrial Proteinsen
dc.subjectProtein Kinase Inhibitorsen
dc.subjectProteomicsen
dc.subjectProtein Bindingen
dc.subjectAurora Kinase Aen
dc.titleConstitutive regulation of mitochondrial morphology by Aurora A kinase depends on a predicted cryptic targeting sequence at the N-terminus.en
dc.typeArticle
prism.issueIdentifier6en
prism.publicationDate2018en
prism.publicationNameOpen biologyen
prism.volume8en
dc.identifier.doi10.17863/CAM.30575
dcterms.dateAccepted2018-05-18en
rioxxterms.versionofrecord10.1098/rsob.170272en
rioxxterms.versionVoR*
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2018-06en
dc.contributor.orcidGrant, Rhys [0000-0003-4027-0972]
dc.contributor.orcidMansfeld, Jörg [0000-0002-0562-8206]
dc.contributor.orcidGlover, David [0000-0003-0956-0103]
dc.contributor.orcidLindon, Ann [0000-0003-3554-2574]
dc.identifier.eissn2046-2441
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idMEDICAL RESEARCH COUNCIL (MR/M01102X/1)
pubs.funder-project-idMRC (982635)
pubs.funder-project-idCancer Research UK (CRUK-A18795)


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International