The effect of cationically-modified phosphorylcholine polymers on human osteoblasts in vitro and their effect on bone formation in vivo
Journal of Materials Science: Materials in Medicine
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Lawton, J., Habib, M., Ma, B., Brooks, R., Best, S., Lewis, A., Rushton, N., & et al. (2017). The effect of cationically-modified phosphorylcholine polymers on human osteoblasts in vitro and their effect on bone formation in vivo. Journal of Materials Science: Materials in Medicine, 28 (144)https://doi.org/10.1007/s10856-017-5958-8
The effect of introducing cationic charge into phosphorylcholine (PC)-based polymers has been investigated in this study with a view to using these materials as coatings to improve bone formation and osseointegration at the bone-implant interface. PC-based polymers, which have been used in a variety of medical devices to improve biocompatibility, are associated with low protein adsorption resulting in reduced complement activation, inflammatory response and cell adhesion. However, in some applications, such as orthopaedics, good integration between the implant and bone is needed to allow the distribution of loading stresses and a bioactive response is required. It has previously been shown that the incorporation of cationic charge into PC-based polymers may increase protein adsorption that stimulates subsequent cell adhesion. In this paper, the effect of cationic charge in PC-based polymers on human osteoblasts (HObs) in vitro and the effect of these polymers on bone formation in the rat tibia was assessed. Increasing PC positive surface charge increased HOb cell adhesion and stimulated increased cell differentiation and the production of calcium phosphate deposits. However, when implanted in bone these materials were at best biotolerant, stimulating the production of fibrous tissue and areas of loosely associated matrix (LAM) around the implant. Their development, as formulated in this study, as bone interfacing implant coatings is therefore not warranted.
The authors would specifically like to thank Biocompatibles UK Ltd for supplying the polymers and for financial and academic support, without which this research would not be possible. Financial assistance from the Engineering and Physical Science Research Council and Biotechnology and Biological Sciences Research Council UK is also acknowledged.
External DOI: https://doi.org/10.1007/s10856-017-5958-8
This record's URL: https://www.repository.cam.ac.uk/handle/1810/283221
Attribution 4.0 International
Licence URL: http://creativecommons.org/licenses/by/4.0/
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