Labeling and identifying cell-specific proteomes in the mouse brain.
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Authors
Krogager, Toke P
Ernst, Russell J
Elliott, Thomas S
Calo, Laura
Beránek, Václav
Ciabatti, Ernesto
Spillantini, Maria Grazia
Tripodi, Marco
Hastings, Michael H
Chin, Jason W
Publication Date
2018-02Journal Title
Nature Biotechnology
ISSN
1087-0156
Publisher
Springer Nature
Volume
36
Issue
2
Pages
156-159
Language
eng
Type
Article
This Version
AM
Metadata
Show full item recordCitation
Krogager, T. P., Ernst, R. J., Elliott, T. S., Calo, L., Beránek, V., Ciabatti, E., Spillantini, M. G., et al. (2018). Labeling and identifying cell-specific proteomes in the mouse brain.. Nature Biotechnology, 36 (2), 156-159. https://doi.org/10.1038/nbt.4056
Abstract
We develop an approach to tag proteomes synthesized by specific cell types in dissociated cortex, brain slices, and the brains of live mice. By viral-mediated expression of an orthogonal pyrrolysyl-tRNA synthetase-tRNAXXX pair in a cell type of interest and providing a non-canonical amino acid with a chemical handle, we selectively label neuronal or glial proteomes. The method enables the identification of proteins from spatially and genetically defined regions of the brain.
Sponsorship
Michael J Fox Foundation (MJFF) (unknown), Parkinson's UK (None), Cure Parkinson's Trust (unknown)
Funder references
Michael J Fox Foundation (MJFF) (unknown)
Parkinson's UK (None)
Cure Parkinson's Trust (unknown)
Identifiers
External DOI: https://doi.org/10.1038/nbt.4056
This record's URL: https://www.repository.cam.ac.uk/handle/1810/283245
Rights
Licence:
http://www.rioxx.net/licenses/all-rights-reserved
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