Labeling and identifying cell-specific proteomes in the mouse brain.

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Authors
Krogager, Toke P
Ernst, Russell J
Elliott, Thomas S
Calo, Laura
Beránek, Václav
Ciabatti, Ernesto
Spillantini, Maria Grazia
Publication Date
2018-02
Journal Title
Nature Biotechnology
ISSN
1087-0156
Publisher
Springer Nature
Volume
36
Issue
2
Pages
156-159
Language
eng
Type
Article
This Version
AM
Metadata
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Citation
Krogager, T. P., Ernst, R. J., Elliott, T. S., Calo, L., Beránek, V., Ciabatti, E., Spillantini, M. G., et al. (2018). Labeling and identifying cell-specific proteomes in the mouse brain.. Nature Biotechnology, 36 (2), 156-159. https://doi.org/10.1038/nbt.4056
Abstract
We develop an approach to tag proteomes synthesized by specific cell types in dissociated cortex, brain slices, and the brains of live mice. By viral-mediated expression of an orthogonal pyrrolysyl-tRNA synthetase-tRNAXXX pair in a cell type of interest and providing a non-canonical amino acid with a chemical handle, we selectively label neuronal or glial proteomes. The method enables the identification of proteins from spatially and genetically defined regions of the brain.
Sponsorship
Michael J Fox Foundation (MJFF) (unknown), Parkinson's UK (None), Cure Parkinson's Trust (unknown)
Funder references
Michael J Fox Foundation (MJFF) (unknown)
Parkinson's UK (None)
Cure Parkinson's Trust (unknown)
Identifiers
External DOI: https://doi.org/10.1038/nbt.4056
This record's URL: https://www.repository.cam.ac.uk/handle/1810/283245
Rights
Licence:
http://www.rioxx.net/licenses/all-rights-reserved
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