Labeling and identifying cell-specific proteomes in the mouse brain.
Krogager, Toke P
Ernst, Russell J
Elliott, Thomas S
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Krogager, T. P., Ernst, R. J., Elliott, T. S., Calo, L., Beránek, V., Ciabatti, E., Spillantini, M., et al. (2018). Labeling and identifying cell-specific proteomes in the mouse brain.. Nature Biotechnology, 36 (2), 156-159. https://doi.org/10.1038/nbt.4056
We develop an approach to tag proteomes synthesized by specific cell types in dissociated cortex, brain slices, and the brains of live mice. By viral-mediated expression of an orthogonal pyrrolysyl-tRNA synthetase-tRNAXXX pair in a cell type of interest and providing a non-canonical amino acid with a chemical handle, we selectively label neuronal or glial proteomes. The method enables the identification of proteins from spatially and genetically defined regions of the brain.
Michael J Fox Foundation (MJFF) (unknown), Parkinson's UK (None), Cure Parkinson's Trust (unknown)
Michael J Fox Foundation (MJFF) (unknown)
Parkinson's UK (G-1102)
Cure Parkinson's Trust (unknown)
External DOI: https://doi.org/10.1038/nbt.4056
This record's URL: https://www.repository.cam.ac.uk/handle/1810/283245