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Distinct Trends of DNA Methylation Patterning in the Innate and Adaptive Immune Systems.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Schuyler, Ronald P 
Merkel, Angelika 
Raineri, Emanuele 
Altucci, Lucia 
Vellenga, Edo 

Abstract

DNA methylation and the localization and post-translational modification of nucleosomes are interdependent factors that contribute to the generation of distinct phenotypes from genetically identical cells. With 112 whole-genome bisulfite sequencing datasets from the BLUEPRINT Epigenome Project, we analyzed the global development of DNA methylation patterns during lineage commitment and maturation of a range of immune system effector cells and the cancers that arise from them. We show clear trends in methylation patterns that are distinct in the innate and adaptive arms of the human immune system, both globally and in relation to consistently positioned nucleosomes. Most notable are a progressive loss of methylation in developing lymphocytes and the consistent occurrence of non-CG methylation in specific cell types. Cancer samples from the two lineages are further polarized, suggesting the involvement of distinct lineage-specific epigenetic mechanisms. We anticipate broad utility for this resource as a basis for further comparative epigenetic analyses.

Description

Keywords

BLUEPRINT, CTCF, DNA methylation, epigenetics, hematopoiesis, nucleosomes, whole-genome bisulfite sequencing, Adaptive Immunity, B-Lymphocytes, Base Sequence, Binding Sites, CCCTC-Binding Factor, DNA Methylation, Dinucleoside Phosphates, Exons, Humans, Immunity, Innate, Lymphocytes, Myeloid Cells, Nucleosomes

Journal Title

Cell Rep

Conference Name

Journal ISSN

2211-1247
2211-1247

Volume Title

17

Publisher

Elsevier BV
Sponsorship
European Commission (282510)
British Heart Foundation (None)
British Heart Foundation (None)
CCF (None)