Show simple item record

dc.contributor.authorLe Pen, Jérémie
dc.contributor.authorJiang, Hongbing
dc.contributor.authorDi Domenico, Tomás
dc.contributor.authorKneuss, Emma
dc.contributor.authorKosałka, Joanna
dc.contributor.authorLeung, Christian
dc.contributor.authorMorgan, Marcos
dc.contributor.authorMuch, Christian
dc.contributor.authorRudolph, Konrad LM
dc.contributor.authorEnright, Anton J
dc.contributor.authorO'Carroll, Dónal
dc.contributor.authorWang, David
dc.contributor.authorMiska, Eric A
dc.date.accessioned2018-10-10T05:16:29Z
dc.date.available2018-10-10T05:16:29Z
dc.date.issued2018-09
dc.identifier.issn1545-9993
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/283341
dc.description.abstractRNA viruses are a major threat to animals and plants. RNA interference (RNAi) and the interferon response provide innate antiviral defense against RNA viruses. Here, we performed a large-scale screen using Caenorhabditis elegans and its natural pathogen the Orsay virus (OrV), and we identified cde-1 as important for antiviral defense. CDE-1 is a homolog of the mammalian TUT4 and TUT7 terminal uridylyltransferases (collectively called TUT4(7)); its catalytic activity is required for its antiviral function. CDE-1 uridylates the 3' end of the OrV RNA genome and promotes its degradation in a manner independent of the RNAi pathway. Likewise, TUT4(7) enzymes uridylate influenza A virus (IAV) mRNAs in mammalian cells. Deletion of TUT4(7) leads to increased IAV mRNA and protein levels. Collectively, these data implicate 3'-terminal uridylation of viral RNAs as a conserved antiviral defense mechanism.
dc.description.sponsorshipCRUK, The Wellcome Trust & ERC
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.subjectAnimals
dc.subjectHumans
dc.subjectCaenorhabditis elegans
dc.subjectRNA Viruses
dc.subjectRNA Nucleotidyltransferases
dc.subjectRNA, Viral
dc.subjectVirus Replication
dc.subjectRNA Interference
dc.subjectImmunity, Innate
dc.subjectTranscriptome
dc.subjectA549 Cells
dc.titleTerminal uridylyltransferases target RNA viruses as part of the innate immune system.
dc.typeArticle
prism.endingPage786
prism.issueIdentifier9
prism.publicationDate2018
prism.publicationNameNat Struct Mol Biol
prism.startingPage778
prism.volume25
dc.identifier.doi10.17863/CAM.30709
dcterms.dateAccepted2018-06-29
rioxxterms.versionofrecord10.1038/s41594-018-0106-9
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-09
dc.contributor.orcidKneuss, Emma [0000-0003-0662-8539]
dc.contributor.orcidMuch, Christian [0000-0002-1644-8200]
dc.contributor.orcidRudolph, Konrad LM [0000-0002-9866-7051]
dc.contributor.orcidEnright, Anton J [0000-0002-6090-3100]
dc.contributor.orcidO'Carroll, Dónal [0000-0002-8626-2217]
dc.contributor.orcidMiska, Eric A [0000-0002-4450-576X]
dc.identifier.eissn1545-9985
rioxxterms.typeJournal Article/Review
pubs.funder-project-idCancer Research Uk (None)
pubs.funder-project-idEuropean Research Council (260688)
pubs.funder-project-idWellcome Trust (104640/Z/14/Z)
pubs.funder-project-idCancer Research UK (18583)
pubs.funder-project-idWellcome Trust (092096/Z/10/Z)
pubs.funder-project-idWellcome Trust (093970/Z/10/Z)
pubs.funder-project-idWellcome Trust (102452/Z/13/Z)
pubs.funder-project-idCancer Research Uk (None)
cam.issuedOnline2018-08-13
rioxxterms.freetoread.startdate2019-02-13


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record