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dc.contributor.authorHeppert, Jennifer K
dc.contributor.authorDickinson, Daniel J
dc.contributor.authorPani, Ariel M
dc.contributor.authorHiggins, Christopher D
dc.contributor.authorSteward, Annette
dc.contributor.authorAhringer, Julie
dc.contributor.authorKuhn, Jeffrey R
dc.contributor.authorGoldstein, Bob
dc.date.accessioned2018-10-10T05:17:05Z
dc.date.available2018-10-10T05:17:05Z
dc.date.issued2016-11-07
dc.identifier.issn1059-1524
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/283362
dc.description.abstractFluorescent protein tags are fundamental tools used to visualize gene products and analyze their dynamics in vivo. Recent advances in genome editing have expedited the precise insertion of fluorescent protein tags into the genomes of diverse organisms. These advances expand the potential of in vivo imaging experiments and facilitate experimentation with new, bright, photostable fluorescent proteins. Most quantitative comparisons of the brightness and photostability of different fluorescent proteins have been made in vitro, removed from biological variables that govern their performance in cells or organisms. To address the gap, we quantitatively assessed fluorescent protein properties in vivo in an animal model system. We generated transgenic Caenorhabditis elegans strains expressing green, yellow, or red fluorescent proteins in embryos and imaged embryos expressing different fluorescent proteins under the same conditions for direct comparison. We found that mNeonGreen was not as bright in vivo as predicted based on in vitro data but is a better tag than GFP for specific kinds of experiments, and we report on optimal red fluorescent proteins. These results identify ideal fluorescent proteins for imaging in vivo in C. elegans embryos and suggest good candidate fluorescent proteins to test in other animal model systems for in vivo imaging experiments.
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherAmerican Society for Cell Biology (ASCB)
dc.rightsPublisher's own licence
dc.subjectAnimals
dc.subjectCaenorhabditis elegans
dc.subjectDisease Models, Animal
dc.subjectLuminescent Proteins
dc.subjectGreen Fluorescent Proteins
dc.subjectFluorescent Dyes
dc.subjectMicroscopy, Fluorescence
dc.subjectOptical Imaging
dc.titleComparative assessment of fluorescent proteins for in vivo imaging in an animal model system.
dc.typeArticle
prism.endingPage3394
prism.issueIdentifier22
prism.publicationDate2016
prism.publicationNameMol Biol Cell
prism.startingPage3385
prism.volume27
dc.identifier.doi10.17863/CAM.30730
dcterms.dateAccepted2016-06-28
rioxxterms.versionofrecord10.1091/mbc.E16-01-0063
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2016-11
dc.contributor.orcidAhringer, Julie [0000-0002-7074-4051]
dc.identifier.eissn1939-4586
rioxxterms.typeJournal Article/Review
pubs.funder-project-idWellcome Trust (101863/Z/13/Z)


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