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dc.contributor.authorThi Nguyen, Diep
dc.contributor.authorShepard, Samuel S
dc.contributor.authorBurke, David
dc.contributor.authorJones, Joyce
dc.contributor.authorThor, Sharmi
dc.contributor.authorNguyen, Long Van
dc.contributor.authorNguyen, Tho Dang
dc.contributor.authorBalish, Amanda
dc.contributor.authorHoang, Dang Nguyen
dc.contributor.authorTo, Thanh Long
dc.contributor.authorIqbal, Munir
dc.contributor.authorWentworth, David E
dc.contributor.authorSpackman, Erica
dc.contributor.authorvan Doorn, H Rogier
dc.contributor.authorDavis, C Todd
dc.contributor.authorBryant, Juliet E
dc.date.accessioned2018-10-10T05:17:22Z
dc.date.available2018-10-10T05:17:22Z
dc.date.issued2018-05-31
dc.identifier.issn2222-1751
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/283372
dc.description.abstractHighly pathogenic avian influenza (HPAI) A(H5N1) viruses pose a significant economic burden to the poultry industry worldwide and have pandemic potential. Poultry vaccination against HPAI A(H5N1) viruses has been an important component of HPAI control measures and has been performed in Vietnam since 2005. To systematically assess antigenic matching of current vaccines to circulating field variants, we produced a panel of chicken and ferret antisera raised against historical and contemporary Vietnamese reference viruses representing clade variants that were detected between 2001 and 2014. The antisera were used for hemagglutination inhibition (HI) assays to generate data sets for analysis by antigenic cartography, allowing for a direct comparison of results from chicken or ferret antisera. HI antigenic maps, developed with antisera from both hosts, revealed varying patterns of antigenic relationships and clustering of viruses that were dependent on the clade of viruses analyzed. Antigenic relationships between existing poultry vaccines and circulating field viruses were also aligned with in vivo protection profiles determined by previously reported vaccine challenge studies. Our results establish the feasibility and utility of HPAI A(H5N1) antigenic characterization using chicken antisera and support further experimental and modeling studies to investigate quantitative relationships between genetic variation, antigenic drift and correlates of poultry vaccine protection in vivo.
dc.format.mediumElectronic
dc.languageeng
dc.publisherInforma UK Limited
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectChick Embryo
dc.subjectAnimals
dc.subjectChickens
dc.subjectFerrets
dc.subjectPoultry Diseases
dc.subjectHemagglutinin Glycoproteins, Influenza Virus
dc.subjectImmune Sera
dc.subjectAntibodies, Viral
dc.subjectHemagglutination Inhibition Tests
dc.subjectPhylogeny
dc.subjectSpecies Specificity
dc.subjectAntigenic Variation
dc.subjectVietnam
dc.subjectFemale
dc.subjectMale
dc.subjectInfluenza A Virus, H5N1 Subtype
dc.subjectInfluenza in Birds
dc.titleAntigenic characterization of highly pathogenic avian influenza A(H5N1) viruses with chicken and ferret antisera reveals clade-dependent variation in hemagglutination inhibition profiles.
dc.typeArticle
prism.issueIdentifier1
prism.publicationDate2018
prism.publicationNameEmerg Microbes Infect
prism.startingPage100
prism.volume7
dc.identifier.doi10.17863/CAM.30740
dcterms.dateAccepted2018-04-18
rioxxterms.versionofrecord10.1038/s41426-018-0100-7
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-05-31
dc.contributor.orcidBurke, David [0000-0001-8830-3951]
dc.contributor.orcidThor, Sharmi [0000-0002-0234-5403]
dc.identifier.eissn2222-1751
rioxxterms.typeJournal Article/Review
pubs.funder-project-idNational Institutes of Health (NIH) (via Mount Sinai School of Medicine (MSSM)) (0258-0513/HHSN272201400008C)
pubs.funder-project-idNational Institutes of Health (NIH) (via Mount Sinai School of Medicine (MSSM)) (HHSN272201400008C)
cam.issuedOnline2018-05-31


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International