MitoQ protects dopaminergic neurons in a 6-OHDA induced PD model by enhancing Mfn2-dependent mitochondrial fusion via activation of PGC-1α.
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Authors
Xi, Ye
Feng, Dayun
Tao, Kai
Wang, Ronglin
Shi, Yajun
Qin, Huaizhou
Yang, Qian
Zhao, Gang
Publication Date
2018-09Journal Title
Biochim Biophys Acta Mol Basis Dis
ISSN
0925-4439
Publisher
Elsevier
Volume
1864
Issue
9 Pt B
Pages
2859-2870
Language
eng
Type
Article
Metadata
Show full item recordCitation
Xi, Y., Feng, D., Tao, K., Wang, R., Shi, Y., Qin, H., Murphy, M., et al. (2018). MitoQ protects dopaminergic neurons in a 6-OHDA induced PD model by enhancing Mfn2-dependent mitochondrial fusion via activation of PGC-1α.. Biochim Biophys Acta Mol Basis Dis, 1864 (9 Pt B), 2859-2870. https://doi.org/10.1016/j.bbadis.2018.05.018
Abstract
Parkinson's disease (PD) is characterized by the degeneration of dopaminergic neurons in the substantia nigra compacta (SNc). Although mitochondrial dysfunction is the critical factor in the pathogenesis of PD, the underlying molecular mechanisms are not well understood, and as a result, effective medical interventions are lacking. Mitochondrial fission and fusion play important roles in the maintenance of mitochondrial function and cell viability. Here, we investigated the effects of MitoQ, a mitochondria-targeted antioxidant, in 6-hydroxydopamine (6-OHDA)-induced in vitro and in vivo PD models. We observed that 6-OHDA enhanced mitochondrial fission by decreasing the expression of Mfn1, Mfn2 and OPA1 as well as by increasing the expression of Drp1 in the dopaminergic (DA) cell line SN4741. Notably, MitoQ treatment particularly upregulated the Mfn2 protein and mRNA levels and promoted mitochondrial fusion in the presence of 6-OHDA in a Mfn2-dependent manner. In addition, MitoQ also stabilized mitochondrial morphology and function in the presence of 6-OHDA, which further suppressed the formation of reactive oxygen species (ROS), as well as ameliorated mitochondrial fragmentation and cellular apoptosis. Moreover, the activation of peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α) was attributed to the upregulation of Mfn2 induced by MitoQ. Consistent with these findings, administration of MitoQ in 6-OHDA-treated mice significantly rescued the decrease of Mfn2 expression and the loss of DA neurons in the SNc. Taken together, our findings suggest that MitoQ protects DA neurons in a 6-OHDA induced PD model by activating PGC-1α to enhance Mfn2-dependent mitochondrial fusion.
Keywords
Mfn2, MitoQ, Mitochondrial fusion, PGC-1α, Parkinson's disease
Sponsorship
This work was supported by National Natural Science Foundation of China, Grant No. 81371365 (G.Z.), National Natural Science Foundation of China, Grant No. 31671060 (Q.Y.) and National Natural Science Foundation of China, Grant No. 81500909 (D.F.)
Funder references
Medical Research Council (MC_UU_00015/3)
Wellcome Trust (110159/Z/15/Z)
Identifiers
External DOI: https://doi.org/10.1016/j.bbadis.2018.05.018
This record's URL: https://www.repository.cam.ac.uk/handle/1810/283465
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