Show simple item record

dc.contributor.authorStewart, Hazelen
dc.contributor.authorBrown, Katherineen
dc.contributor.authorDinan, Adamen
dc.contributor.authorIrigoyen, Nereaen
dc.contributor.authorSnijder, Eric Jen
dc.contributor.authorFirth, Andrewen
dc.date.accessioned2018-10-10T10:44:53Z
dc.date.available2018-10-10T10:44:53Z
dc.date.issued2018-09en
dc.identifier.issn0022-538X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/283526
dc.description.abstractThe genus Torovirus (subfamily Torovirinae, family Coronaviridae, order Nidovirales) encompasses a range of species that infect domestic ungulates including cattle, sheep, goats, pigs and horses, causing an acute self-limiting gastroenteritis. Using the prototype species equine torovirus (EToV) we performed parallel RNA sequencing (RNA-seq) and ribosome profiling (Ribo-seq) to analyse the relative expression levels of the known torovirus proteins and transcripts, chimaeric sequences produced via discontinuous RNA synthesis (a characteristic of the nidovirus replication cycle) and changes in host transcription and translation as a result of EToV infection. RNA sequencing confirmed that EToV utilises a unique combination of discontinuous and non-discontinuous RNA synthesis to produce its subgenomic RNAs; indeed, we identified transcripts arising from both mechanisms that would result in sgRNAs encoding the nucleocapsid. Our ribosome profiling analysis revealed that ribosomes efficiently translate two novel CUG-initiated ORFs, located within the so-called 5’ UTR. We have termed the resulting proteins U1 and U2. Comparative genomic analysis confirmed that these ORFs are conserved across all available torovirus sequences and the inferred amino acid sequences are subject to purifying selection, indicating that U1 and U2 are functionally relevant. This study provides the first high-resolution analysis of transcription and translation in this neglected group of livestock pathogens.
dc.format.mediumElectronic-Printen
dc.languageengen
dc.publisherAmerican Society for Microbiology
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCells, Cultureden
dc.subjectAnimalsen
dc.subjectHorsesen
dc.subjectTorovirusen
dc.subjectViral Proteinsen
dc.subjectVirus Cultivationen
dc.subjectGene Expression Profilingen
dc.subjectSequence Analysis, RNAen
dc.subjectProtein Biosynthesisen
dc.subjectTranscription, Geneticen
dc.subjectHost-Pathogen Interactionsen
dc.titleTranscriptional and Translational Landscape of Equine Torovirus.en
dc.typeArticle
prism.issueIdentifier17en
prism.publicationDate2018en
prism.publicationNameJournal of virologyen
prism.volume92en
dc.identifier.doi10.17863/CAM.30889
dcterms.dateAccepted2018-06-13en
rioxxterms.versionofrecord10.1128/jvi.00589-18en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2018-09en
dc.contributor.orcidBrown, Katherine [0000-0002-8400-6922]
dc.contributor.orcidDinan, Adam [0000-0003-2812-1616]
dc.contributor.orcidIrigoyen, Nerea [0000-0001-6346-3369]
dc.contributor.orcidSnijder, Eric J [0000-0003-3297-2309]
dc.contributor.orcidFirth, Andrew [0000-0002-7986-9520]
dc.identifier.eissn1098-5514
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWELLCOME TRUST (106207/Z/14/Z)
pubs.funder-project-idEuropean Commission Horizon 2020 (H2020) ERC (646891)


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International