Neutral lineage tracing of proliferative embryonic and adult mammary stem/progenitor cells.
Development (Cambridge, England)
Company of Biologists
MetadataShow full item record
Lloyd-Lewis, B., Davis, F. M., Harris, O., Hitchcock, J. R., & Watson, C. (2018). Neutral lineage tracing of proliferative embryonic and adult mammary stem/progenitor cells.. Development (Cambridge, England), 145 (14)https://doi.org/10.1242/dev.164079
Mammary gland development occurs over multiple phases, beginning in the mammalian embryo and continuing throughout reproductive life. The remarkable morphogenetic capacity of the mammary gland at each stage of development is attributed to the activities of distinct populations of mammary stem cells (MaSCs) and progenitor cells. However, the relationship between embryonic and adult MaSCs, and their fate during different waves of mammary gland morphogenesis, remains unclear. Employing a neutral, low-density genetic labelling strategy, we characterised the contribution of proliferative stem/progenitor cells to embryonic, pubertal and reproductive mammary gland development. Our findings further support a model of lineage-restriction of MaSCs in the postnatal mammary gland, and highlight extensive redundancy and heterogeneity within the adult stem/progenitor cell pool. Furthermore, our data suggest extensive multiplicity in their fetal precursors that give rise to the primordial mammary epithelium before birth. In addition, using a single-cell labelling approach, we revealed the extraordinary capacity of a single embryonic MaSC to contribute to postnatal ductal development. Together, these findings provide tantalising new insights into the disparate and stage-specific contribution of distinct stem/progenitor cells to mammary gland development
Mammary Glands, Animal, Animals, Mice, Cell Proliferation, Cell Lineage, Morphogenesis, Embryonic Development, Sexual Maturation, Adult Stem Cells, Single-Cell Analysis, Mouse Embryonic Stem Cells
This work was supported by the Medical Research Council [MR/J001023/1 to C.J.W. and B.L-L and MR/N022963/1 to C.J.W. and J.R.H], National Health and Medical Research Council [1071074 and 1141008 to F.M.D] and Wellcome Trust [105377/Z/14/Z to O.B.H].
WELLCOME TRUST (105377/Z/14/Z)
External DOI: https://doi.org/10.1242/dev.164079
This record's URL: https://www.repository.cam.ac.uk/handle/1810/283549
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/