Neutral lineage tracing of proliferative embryonic and adult mammary stem/progenitor cells.
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Authors
Hitchcock, Jessica R
Publication Date
2018-07-25Journal Title
Development
ISSN
0950-1991
Publisher
The Company of Biologists
Volume
145
Issue
14
Language
eng
Type
Article
Physical Medium
Electronic
Metadata
Show full item recordCitation
Lloyd-Lewis, B., Davis, F. M., Harris, O. B., Hitchcock, J. R., & Watson, C. J. (2018). Neutral lineage tracing of proliferative embryonic and adult mammary stem/progenitor cells.. Development, 145 (14) https://doi.org/10.1242/dev.164079
Abstract
Mammary gland development occurs over multiple phases, beginning in the mammalian embryo and continuing throughout reproductive life. The remarkable morphogenetic capacity of the mammary gland at each stage of development is attributed to the activities of distinct populations of mammary stem cells (MaSCs) and progenitor cells. However, the relationship between embryonic and adult MaSCs, and their fate during different waves of mammary gland morphogenesis, remains unclear. By employing a neutral, low-density genetic labelling strategy, we characterised the contribution of proliferative stem/progenitor cells to embryonic, pubertal and reproductive mammary gland development. Our findings further support a model of lineage restriction of MaSCs in the postnatal mammary gland, and highlight extensive redundancy and heterogeneity within the adult stem/progenitor cell pool. Furthermore, our data suggest extensive multiplicity in their foetal precursors that give rise to the primordial mammary epithelium before birth. In addition, using a single-cell labelling approach, we revealed the extraordinary capacity of a single embryonic MaSC to contribute to postnatal ductal development. Together, these findings provide tantalising new insights into the disparate and stage-specific contribution of distinct stem/progenitor cells to mammary gland development.
Keywords
Adult mammary stem cells, Embryonic mammary stem cells, Lineage tracing, Mammary gland development, Adult Stem Cells, Animals, Cell Lineage, Cell Proliferation, Embryonic Development, Mammary Glands, Animal, Mice, Morphogenesis, Mouse Embryonic Stem Cells, Sexual Maturation, Single-Cell Analysis
Sponsorship
This work was supported by the Medical Research Council [MR/J001023/1 to C.J.W. and B.L-L and MR/N022963/1 to C.J.W. and J.R.H], National Health and Medical Research Council [1071074 and 1141008 to F.M.D] and Wellcome Trust [105377/Z/14/Z to O.B.H].
Funder references
Medical Research Council (MR/J001023/1)
Medical Research Council (MR/N022963/1)
Medical Research Council (MC_PC_12009)
Wellcome Trust (105377/Z/14/Z)
Identifiers
External DOI: https://doi.org/10.1242/dev.164079
This record's URL: https://www.repository.cam.ac.uk/handle/1810/283549
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