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dc.contributor.authorMiyamoto, Kei
dc.contributor.authorNguyen, Khoi T
dc.contributor.authorAllen, George E
dc.contributor.authorJullien, Jerome
dc.contributor.authorKumar, Dinesh
dc.contributor.authorOtani, Tomoki
dc.contributor.authorBradshaw, Charles
dc.contributor.authorLivesey, Frederick
dc.contributor.authorKellis, Manolis
dc.contributor.authorGurdon, John
dc.date.accessioned2018-10-10T10:45:40Z
dc.date.available2018-10-10T10:45:40Z
dc.date.issued2018-07-10
dc.identifier.issn2211-1247
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/283552
dc.description.abstractOocytes have a remarkable ability to reactivate silenced genes in somatic cells. However, it is not clear how the chromatin architecture of somatic cells affects this transcriptional reprogramming. Here, we investigated the relationship between the chromatin opening and transcriptional activation. We reveal changes in chromatin accessibility and their relevance to transcriptional reprogramming after transplantation of somatic nuclei into Xenopus oocytes. Genes that are silenced, but have pre-existing open transcription start sites in donor cells, are prone to be activated after nuclear transfer, suggesting that the chromatin signature of somatic nuclei influences transcriptional reprogramming. There are also activated genes associated with new open chromatin sites, and transcription factors in oocytes play an important role in transcriptional reprogramming from such genes. Finally, we show that genes resistant to reprogramming are associated with closed chromatin configurations. We conclude that chromatin accessibility is a central factor for successful transcriptional reprogramming in oocytes.
dc.format.mediumPrint
dc.languageeng
dc.publisherElsevier BV
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectOocytes
dc.subjectChromatin
dc.subjectFibroblasts
dc.subjectAnimals
dc.subjectXenopus laevis
dc.subjectMice
dc.subjectTransposases
dc.subjectTranscription Factors
dc.subjectSequence Analysis, DNA
dc.subjectTranscription, Genetic
dc.subjectTranscription Initiation Site
dc.subjectPromoter Regions, Genetic
dc.subjectTranscriptional Activation
dc.subjectCellular Reprogramming
dc.titleChromatin Accessibility Impacts Transcriptional Reprogramming in Oocytes.
dc.typeArticle
prism.endingPage311
prism.issueIdentifier2
prism.publicationDate2018
prism.publicationNameCell Rep
prism.startingPage304
prism.volume24
dc.identifier.doi10.17863/CAM.30915
dcterms.dateAccepted2018-06-07
rioxxterms.versionofrecord10.1016/j.celrep.2018.06.030
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-07
dc.contributor.orcidJullien, Jerome [0000-0002-7868-0021]
dc.contributor.orcidBradshaw, Charles [0000-0002-3528-458X]
dc.contributor.orcidLivesey, Frederick [0000-0001-6128-3372]
dc.contributor.orcidGurdon, John [0000-0002-5621-3799]
dc.identifier.eissn2211-1247
rioxxterms.typeJournal Article/Review
pubs.funder-project-idWellcome Trust (101050/Z/13/Z)
pubs.funder-project-idWellcome Trust (092096/Z/10/Z)
pubs.funder-project-idMedical Research Council (MR/K011022/1)
pubs.funder-project-idCancer Research Uk (None)
pubs.funder-project-idMedical Research Council (MR/L023784/2)
pubs.funder-project-idMedical Research Council (MR/L023784/1)


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International