The cytotoxic effect of TiF4 and NaF on fibroblasts is influenced by the experimental model, fluoride concentration and exposure time.
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Authors
Salomão, Priscila Maria Aranda
Oliveira, Flávia Amadeu de
Rodrigues, Paula Danielle
Al-Ahj, Luana Polioni
Gasque, Kellen Cristina da Silva
Jeggle, Pia
Buzalaf, Marilia Afonso Rabelo
Oliveira, Rodrigo Cardoso de
Edwardson, Michael
Publication Date
2017Journal Title
PLoS One
ISSN
1932-6203
Publisher
Public Library of Science (PLoS)
Volume
12
Issue
6
Pages
e0179471
Language
eng
Type
Article
Physical Medium
Electronic-eCollection
Metadata
Show full item recordCitation
Salomão, P. M. A., Oliveira, F. A. d., Rodrigues, P. D., Al-Ahj, L. P., Gasque, K. C. d. S., Jeggle, P., Buzalaf, M. A. R., et al. (2017). The cytotoxic effect of TiF4 and NaF on fibroblasts is influenced by the experimental model, fluoride concentration and exposure time.. PLoS One, 12 (6), e0179471. https://doi.org/10.1371/journal.pone.0179471
Abstract
OBJECTIVE: Titanium tetrafluoride (TiF4) has shown promising effect in preventing tooth lesions. Therefore, we compared the cytotoxicity of TiF4 with sodium fluoride (NaF) (already applied in Dentistry) considering different fluoride concentrations, pH values and experimental models. MATERIALS AND METHODS: Step 1) NIH/3T3 fibroblasts were exposed to mediums containing NaF or TiF4 (from 0.15 to 2.45% F), both at native and adjusted pH, for 6 h. Step 2) NIH/3T3 were exposed to NaF or TiF4 varnishes with 0.95, 1.95 or 2.45% F (native pH), for 6, 12 or 24 h. We applied MTT (1st and 2nd steps) and Hoescht/PI stain (2nd step) assays. Step 3) NIH/3T3 were exposed to NaF or TiF4 varnish (2.45% F), at native pH, for 6 or 12 h. The cell stiffness was measured by atomic force microscopy (AFM). RESULTS: Step 1) All cells exposed to NaF or TiF4 mediums died, regardless of the F concentration and pH. Step 2) Both varnishes, at 1.90 and 2.45% F, reduced cell viability by similar extents (33-86% at 6 h, 35-93% at 12 h, and 87-98% at 24 h) compared with control, regardless of the type of fluoride. Varnishes with 0.95% F did not differ from control. Step 3) TiF4 and NaF reduced cell stiffness to a similar extent, but only TiF4 differed from control at 6 h. CONCLUSIONS: Based on the results of the 3 experimental steps, we conclude that TiF4 and NaF have similar cytotoxicity. The cytotoxicity was dependent on F concentration and exposure time. This result gives support for testing the effect of TiF4 varnish in vivo.
Keywords
NIH 3T3 Cells, Fibroblasts, Animals, Mice, Fluorides, Titanium, Sodium Fluoride, Cariostatic Agents, Microscopy, Atomic Force, Cell Survival, Dose-Response Relationship, Drug, Hydrogen-Ion Concentration, Models, Theoretical, Time Factors, Cell Physiological Phenomena
Identifiers
External DOI: https://doi.org/10.1371/journal.pone.0179471
This record's URL: https://www.repository.cam.ac.uk/handle/1810/284194
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