Expression of SULTR2;2, encoding a low-affinity sulphur transporter, in the Arabidopsis bundle sheath and vein cells is mediated by a positive regulator.
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Authors
Kirschner, Sandra
Woodfield, Helen
Prusko, Katharina
Koczor, Maria
Gowik, Udo
Hibberd, Julian M
Westhoff, Peter
Publication Date
2018-09-14Journal Title
J Exp Bot
ISSN
0022-0957
Publisher
Oxford University Press (OUP)
Volume
69
Issue
20
Pages
4897-4906
Language
eng
Type
Article
Physical Medium
Print
Metadata
Show full item recordCitation
Kirschner, S., Woodfield, H., Prusko, K., Koczor, M., Gowik, U., Hibberd, J. M., & Westhoff, P. (2018). Expression of SULTR2;2, encoding a low-affinity sulphur transporter, in the Arabidopsis bundle sheath and vein cells is mediated by a positive regulator.. J Exp Bot, 69 (20), 4897-4906. https://doi.org/10.1093/jxb/ery263
Abstract
The bundle sheath provides a conduit linking veins and mesophyll cells. In the C3 plant Arabidopsis thaliana, it also plays important roles in oxidative stress and sulphur metabolism. However, the mechanisms responsible for the patterns of gene expression that underpin these metabolic specializations are poorly understood. Here, we used the Arabidopsis SULTR2;2 gene as a model to better understand mechanisms that restrict expression to the bundle sheath. Deletion analysis indicated that the SULTR2;2 promoter contains a short region necessary for expression in the bundle sheath and veins. This sequence acts as a positive regulator and is tolerant to multiple consecutive deletions indicating considerable redundancy in the cis-elements involved. It is highly conserved in SULTR2;2 genes of the Brassicaceae and is functional in the distantly related C4 species Flaveria bidentis that belongs to the Asteraceae. We conclude that expression of SULTR2;2 in the bundle sheath and veins is underpinned by a highly redundant sequence that likely represents an ancient and conserved mechanism found in families as diverse as the Asteraceae and Brassicaceae.
Keywords
Arabidopsis, Base Sequence, Gene Expression Regulation, Plant, Plant Leaves, Plant Vascular Bundle, Sequence Alignment
Sponsorship
BBSRC PhD studentship
Deutsche Forschungsgemeinschaft
Identifiers
External DOI: https://doi.org/10.1093/jxb/ery263
This record's URL: https://www.repository.cam.ac.uk/handle/1810/284473
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