Identification of an algal xylan synthase indicates that there is functional orthology between algal and plant cell wall biosynthesis.
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Authors
Jensen, Jacob Krüger
Busse-Wicher, Marta
Poulsen, Christian Peter
Fangel, Jonatan Ulrik
Smith, Peter James
Yang, Jeong-Yeh
Peña, Maria-Jesus
Dinesen, Malene Hessellund
Martens, Helle Juel
Melkonian, Michael
Wong, Gane Ka-Shu
Moremen, Kelley W
Wilkerson, Curtis Gene
Scheller, Henrik Vibe
Ulvskov, Peter
Urbanowicz, Breeanna Rae
Harholt, Jesper
Publication Date
2018-05Journal Title
New Phytol
ISSN
0028-646X
Publisher
Wiley
Volume
218
Issue
3
Pages
1049-1060
Language
eng
Type
Article
Physical Medium
Print-Electronic
Metadata
Show full item recordCitation
Jensen, J. K., Busse-Wicher, M., Poulsen, C. P., Fangel, J. U., Smith, P. J., Yang, J., Peña, M., et al. (2018). Identification of an algal xylan synthase indicates that there is functional orthology between algal and plant cell wall biosynthesis.. New Phytol, 218 (3), 1049-1060. https://doi.org/10.1111/nph.15050
Abstract
Insights into the evolution of plant cell walls have important implications for comprehending these diverse and abundant biological structures. In order to understand the evolving structure-function relationships of the plant cell wall, it is imperative to trace the origin of its different components. The present study is focused on plant 1,4-β-xylan, tracing its evolutionary origin by genome and transcriptome mining followed by phylogenetic analysis, utilizing a large selection of plants and algae. It substantiates the findings by heterologous expression and biochemical characterization of a charophyte alga xylan synthase. Of the 12 known gene classes involved in 1,4-β-xylan formation, XYS1/IRX10 in plants, IRX7, IRX8, IRX9, IRX14 and GUX occurred for the first time in charophyte algae. An XYS1/IRX10 ortholog from Klebsormidium flaccidum, designated K. flaccidumXYLAN SYNTHASE-1 (KfXYS1), possesses 1,4-β-xylan synthase activity, and 1,4-β-xylan occurs in the K. flaccidum cell wall. These data suggest that plant 1,4-β-xylan originated in charophytes and shed light on the origin of one of the key cell wall innovations to occur in charophyte algae, facilitating terrestrialization and emergence of polysaccharide-based plant cell walls.
Keywords
Cell Wall, Humans, Pentosyltransferases, Evolution, Molecular, Phylogeny, Amino Acid Motifs, Biosynthetic Pathways, HEK293 Cells, Charophyceae, Plant Cells
Sponsorship
Leverhulme Trust (RP2013-SL-008)
Identifiers
External DOI: https://doi.org/10.1111/nph.15050
This record's URL: https://www.repository.cam.ac.uk/handle/1810/284601
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