Hyperpolarized 13 C spectroscopic imaging using single-shot 3D sequences with unpaired adiabatic refocusing pulses.
NMR in biomedicine
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Wang, J., Hesketh, R., Wright, A. J., & Brindle, K. (2018). Hyperpolarized 13 C spectroscopic imaging using single-shot 3D sequences with unpaired adiabatic refocusing pulses.. NMR in biomedicine, 31 (11), e4004. https://doi.org/10.1002/nbm.4004
Hyperpolarized MRI with 13C-labeled metabolites has enabled metabolic imaging of tumors in vivo. The heterogeneous nature of tumors and the limited lifetime of the hyperpolarization require high resolution, both temporally and spatially. We describe two sequences that make more efficient use of the 13C polarization than previously described single shot 3D sequences. With these sequences, the target metabolite resonances were excited using spectral-spatial pulses and the data acquired using spiral readouts from a series of echoes created using a fast spin echo sequence employing adiabatic 180° pulses. The third dimension was encoded with blipped gradients applied in an interleaved order to the echo train. Adiabatic inversion pulses applied in the absence of slice selection gradients allowed acquisition of signal from odd echoes, formed by unpaired adiabatic pulses, as well as from even echoes. The sequences were tested on tumor-bearing mice following intravenous injection of hyperpolarized [1-13C]pyruvate. [1- 13C]pyruvate and [1-13C]lactate images were acquired in vivo with 4 x 4 x 2 cm3 FOV and 32 x 32 x 16 matrix, leading to a nominal resolution of 1.25 x 1.25 x 1.25 mm3 and an effective resolution of 1.25 x 1.25 x 4.5 mm3 when the z-direction PSF was taken into account. The acquisition of signal from more echoes also allowed for an improvement in the signal-to-noise ratio for resonances with longer T2 relaxation times. The pulse sequences described here produced hyperpolarized 13C images with improved resolution and signal-to-noise when compared to similar sequences described previously.
Animals, Mice, Lactic Acid, Pyruvic Acid, Spin Labels, Imaging, Three-Dimensional, Phantoms, Imaging, Algorithms, Signal Processing, Computer-Assisted, Carbon-13 Magnetic Resonance Spectroscopy
Cancer Research UK Programme grant (17242) to KMB and by the CRUK-EPSRC Imaging Centre in Cambridge and Manchester (16465). JW as in part supported by supported, in part, by a grant from the Danish Strategic Research Council (LIFE-DNP: Hyperpolarized magnetic resonance for in vivo quantification of lipid, sugar and amino acid metabolism in lifestyle related diseases).
Cancer Research UK (CB4100)
Aarhus University (Source: Data Science Research Centre (DSRC)) (15952)
Cancer Research UK (16465)
External DOI: https://doi.org/10.1002/nbm.4004
This record's URL: https://www.repository.cam.ac.uk/handle/1810/284610
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/