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dc.contributor.authorJohanson, Timothy M
dc.contributor.authorCoughlan, Hannah D
dc.contributor.authorLun, Aaron
dc.contributor.authorBediaga, Naiara G
dc.contributor.authorNaselli, Gaetano
dc.contributor.authorGarnham, Alexandra L
dc.contributor.authorHarrison, Leonard C
dc.contributor.authorSmyth, Gordon K
dc.contributor.authorAllan, Rhys S
dc.date.accessioned2018-11-07T00:30:24Z
dc.date.available2018-11-07T00:30:24Z
dc.date.issued2018-06
dc.identifier.issn1553-7390
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/284678
dc.description.abstractIt has been proposed that interactions between mammalian chromosomes, or transchromosomal interactions (also known as kissing chromosomes), regulate gene expression and cell fate determination. Here we aimed to identify novel transchromosomal interactions in immune cells by high-resolution genome-wide chromosome conformation capture. Although we readily identified stable interactions in cis, and also between centromeres and telomeres on different chromosomes, surprisingly we identified no gene regulatory transchromosomal interactions in either mouse or human cells, including previously described interactions. We suggest that advances in the chromosome conformation capture technique and the unbiased nature of this approach allow more reliable capture of interactions between chromosomes than previous methods. Overall our findings suggest that stable transchromosomal interactions that regulate gene expression are not present in mammalian immune cells and that lineage identity is governed by cis, not trans chromosomal interactions.
dc.format.mediumElectronic-eCollection
dc.languageeng
dc.publisherPublic Library of Science (PLoS)
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectChromosomes, Mammalian
dc.subjectChromatin
dc.subjectAnimals
dc.subjectMice, Inbred C57BL
dc.subjectMammals
dc.subjectHumans
dc.subjectMice
dc.subjectDNA
dc.subjectFlow Cytometry
dc.subjectImmunity, Cellular
dc.subjectGene Expression Regulation
dc.subjectNucleic Acid Conformation
dc.subjectGenome
dc.subjectStereoisomerism
dc.subjectMale
dc.titleGenome-wide analysis reveals no evidence of trans chromosomal regulation of mammalian immune development.
dc.typeArticle
prism.issueIdentifier6
prism.publicationDate2018
prism.publicationNamePLoS Genet
prism.startingPagee1007431
prism.volume14
dc.identifier.doi10.17863/CAM.32051
dcterms.dateAccepted2018-05-18
rioxxterms.versionofrecord10.1371/journal.pgen.1007431
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-06-08
dc.contributor.orcidJohanson, Timothy M [0000-0003-4605-6416]
dc.contributor.orcidCoughlan, Hannah D [0000-0002-2392-9942]
dc.contributor.orcidLun, Aaron [0000-0002-3564-4813]
dc.contributor.orcidSmyth, Gordon K [0000-0001-9221-2892]
dc.identifier.eissn1553-7404
rioxxterms.typeJournal Article/Review
cam.issuedOnline2018-06-08


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International