Labeling and identifying cell-type-specific proteomes in the mouse brain
Accepted version
Peer-reviewed
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Authors
Spillantini, MG
Abstract
We develop an approach to tag proteomes synthesized by specific cell-types in dissociated cortex, brain slices, and the brain of live mice. By viral-mediated expression of an orthogonal pyrrolysyl- tRNA synthetase/tRNAXXX pair in a cell type of interest and providing a non-canonical amino acid with a chemical handle, we selectively label neuronal or glial proteomes. The method enables the identification of proteins from spatially and genetically defined regions of the brain.
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Journal Title
Nature Biotechnology
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Journal ISSN
1087-0156
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Publisher
Springer Nature
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Sponsorship
National Centre for the Replacement Refinement and Reduction of Animals in Research (NC/L000741/1)
Cure Parkinson's Trust (unknown)
Alzheimer's Research UK (ARUK-EXT2015B-2)
Cure Parkinson's Trust (unknown)
Alzheimer's Research UK (ARUK-EXT2015B-2)
MRC, ARUK, CurePD, NC3Rs