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Labeling and identifying cell-type-specific proteomes in the mouse brain

Accepted version
Peer-reviewed

Type

Article

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Authors

Spillantini, MG 

Abstract

We develop an approach to tag proteomes synthesized by specific cell-types in dissociated cortex, brain slices, and the brain of live mice. By viral-mediated expression of an orthogonal pyrrolysyl- tRNA synthetase/tRNAXXX pair in a cell type of interest and providing a non-canonical amino acid with a chemical handle, we selectively label neuronal or glial proteomes. The method enables the identification of proteins from spatially and genetically defined regions of the brain.

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Keywords

Journal Title

Nature Biotechnology

Conference Name

Journal ISSN

1087-0156

Volume Title

Publisher

Springer Nature
Sponsorship
National Centre for the Replacement Refinement and Reduction of Animals in Research (NC/L000741/1)
Cure Parkinson's Trust (unknown)
Alzheimer's Research UK (ARUK-EXT2015B-2)
MRC, ARUK, CurePD, NC3Rs