Human axial progenitors generate trunk neural crest cells in vitro.
View / Open Files
Authors
Granata, Ilaria
Wind, Matthew
Stout, Erin
Thompson, Oliver
Neumann, Katrin
Stavish, Dylan
Heath, Paul R
Ortmann, Daniel
Gouti, Mina
Johnson, Stuart L
Placzek, Marysia
Guarracino, Mario R
Andrews, Peter W
Publication Date
2018-08-10Journal Title
Elife
ISSN
2050-084X
Publisher
eLife Sciences Publications, Ltd
Volume
7
Language
eng
Type
Article
Physical Medium
Electronic
Metadata
Show full item recordCitation
Frith, T. J., Granata, I., Wind, M., Stout, E., Thompson, O., Neumann, K., Stavish, D., et al. (2018). Human axial progenitors generate trunk neural crest cells in vitro.. Elife, 7 https://doi.org/10.7554/eLife.35786
Abstract
The neural crest (NC) is a multipotent embryonic cell population that generates distinct cell types in an axial position-dependent manner. The production of NC cells from human pluripotent stem cells (hPSCs) is a valuable approach to study human NC biology. However, the origin of human trunk NC remains undefined and current in vitro differentiation strategies induce only a modest yield of trunk NC cells. Here we show that hPSC-derived axial progenitors, the posteriorly-located drivers of embryonic axis elongation, give rise to trunk NC cells and their derivatives. Moreover, we define the molecular signatures associated with the emergence of human NC cells of distinct axial identities in vitro. Collectively, our findings indicate that there are two routes toward a human post-cranial NC state: the birth of cardiac and vagal NC is facilitated by retinoic acid-induced posteriorisation of an anterior precursor whereas trunk NC arises within a pool of posterior axial progenitors.
Keywords
Cells, Cultured, Pluripotent Stem Cells, Neural Crest, Humans, Cell Differentiation, Biomarkers
Sponsorship
Medical Research Council (MC_PC_12009)
Identifiers
External DOI: https://doi.org/10.7554/eLife.35786
This record's URL: https://www.repository.cam.ac.uk/handle/1810/284966
Statistics
Total file downloads (since January 2020). For more information on metrics see the
IRUS guide.
Recommended or similar items
The current recommendation prototype on the Apollo Repository will be turned off on 03 February 2023. Although the pilot has been fruitful for both parties, the service provider IKVA is focusing on horizon scanning products and so the recommender service can no longer be supported. We recognise the importance of recommender services in supporting research discovery and are evaluating offerings from other service providers. If you would like to offer feedback on this decision please contact us on: support@repository.cam.ac.uk