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dc.contributor.authorFrith, Thomas Jr
dc.contributor.authorGranata, Ilaria
dc.contributor.authorWind, Matthew
dc.contributor.authorStout, Erin
dc.contributor.authorThompson, Oliver
dc.contributor.authorNeumann, Katrin
dc.contributor.authorStavish, Dylan
dc.contributor.authorHeath, Paul R
dc.contributor.authorOrtmann, Daniel
dc.contributor.authorHackland, James Os
dc.contributor.authorAnastassiadis, Konstantinos
dc.contributor.authorGouti, Mina
dc.contributor.authorBriscoe, James
dc.contributor.authorWilson, Valerie
dc.contributor.authorJohnson, Stuart L
dc.contributor.authorPlaczek, Marysia
dc.contributor.authorGuarracino, Mario R
dc.contributor.authorAndrews, Peter W
dc.contributor.authorTsakiridis, Anestis
dc.date.accessioned2018-11-13T00:30:52Z
dc.date.available2018-11-13T00:30:52Z
dc.date.issued2018-08-10
dc.identifier.issn2050-084X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/284966
dc.description.abstractThe neural crest (NC) is a multipotent embryonic cell population that generates distinct cell types in an axial position-dependent manner. The production of NC cells from human pluripotent stem cells (hPSCs) is a valuable approach to study human NC biology. However, the origin of human trunk NC remains undefined and current in vitro differentiation strategies induce only a modest yield of trunk NC cells. Here we show that hPSC-derived axial progenitors, the posteriorly-located drivers of embryonic axis elongation, give rise to trunk NC cells and their derivatives. Moreover, we define the molecular signatures associated with the emergence of human NC cells of distinct axial identities in vitro. Collectively, our findings indicate that there are two routes toward a human post-cranial NC state: the birth of cardiac and vagal NC is facilitated by retinoic acid-induced posteriorisation of an anterior precursor whereas trunk NC arises within a pool of posterior axial progenitors.
dc.format.mediumElectronic
dc.languageeng
dc.publishereLife Sciences Publications, Ltd
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCells, Cultured
dc.subjectPluripotent Stem Cells
dc.subjectNeural Crest
dc.subjectHumans
dc.subjectCell Differentiation
dc.subjectBiomarkers
dc.titleHuman axial progenitors generate trunk neural crest cells in vitro.
dc.typeArticle
prism.publicationDate2018
prism.publicationNameElife
prism.volume7
dc.identifier.doi10.17863/CAM.32337
dcterms.dateAccepted2018-08-09
rioxxterms.versionofrecord10.7554/eLife.35786
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-08-10
dc.contributor.orcidFrith, Thomas Jr [0000-0002-6078-5466]
dc.contributor.orcidHackland, James Os [0000-0001-7087-9995]
dc.contributor.orcidAnastassiadis, Konstantinos [0000-0002-9814-0559]
dc.contributor.orcidBriscoe, James [0000-0002-1020-5240]
dc.contributor.orcidWilson, Valerie [0000-0003-4182-5159]
dc.contributor.orcidTsakiridis, Anestis [0000-0002-2184-2990]
dc.identifier.eissn2050-084X
rioxxterms.typeJournal Article/Review
pubs.funder-project-idMedical Research Council (MC_PC_12009)
cam.issuedOnline2018-08-10


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International