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dc.contributor.authorYu, Li
dc.contributor.authorLyczakowski, Jan J
dc.contributor.authorPereira, Caroline S
dc.contributor.authorKotake, Toshihisa
dc.contributor.authorYu, Xiaolan
dc.contributor.authorLi, An
dc.contributor.authorMogelsvang, Soren
dc.contributor.authorSkaf, Munir S
dc.contributor.authorDupree, Paul
dc.date.accessioned2018-11-14T00:31:24Z
dc.date.available2018-11-14T00:31:24Z
dc.date.issued2018-11
dc.identifier.issn0032-0889
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/285049
dc.description.abstractThe interaction between mannan polysaccharides and cellulose microfibrils contributes to cell wall properties in some vascular plants, but the molecular arrangement of mannan in the cell wall and the nature of the molecular bonding between mannan and cellulose remain unknown. Previous studies have shown that mannan is important in maintaining Arabidopsis (Arabidopsis thaliana) seed mucilage architecture, and that Cellulose Synthase-Like A2 (CSLA2) synthesizes a glucomannan backbone, which Mannan α-Galactosyl Transferase1 (MAGT1/GlycosylTransferase-Like6/Mucilage Related10) might decorate with single α-Gal branches. Here, we investigated the ratio and sequence of Man and Glc and the arrangement of Gal residues in Arabidopsis mucilage mannan using enzyme sequential digestion, carbohydrate gel electrophoresis, and mass spectrometry. We found that seed mucilage galactoglucomannan has a backbone consisting of the repeating disaccharide [4)-β-Glc-(1,4)-β-Man-(1,], and most of the Man residues in the backbone are substituted by single α-1,6-Gal. CSLA2 is responsible for the synthesis of this patterned glucomannan backbone and MAGT1 catalyses the addition of α-Gal. In vitro activity assays revealed that MAGT1 transferred α-Gal from UDP-Gal only to Man residues within the CSLA2 patterned glucomannan backbone acceptor. These results indicate that CSLAs and galactosyltransferases are able to make precisely defined galactoglucomannan structures. Molecular dynamics simulations suggested this patterned galactoglucomannan is able to bind stably to some hydrophilic faces and to hydrophobic faces of cellulose microfibrils. A specialization of the biosynthetic machinery to make galactoglucomannan with a patterned structure may therefore regulate the mode of binding of this hemicellulose to cellulose fibrils.
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherOxford University Press (OUP)
dc.subjectCell Wall
dc.subjectArabidopsis
dc.subjectSeeds
dc.subjectCellulose
dc.subjectGlycosyltransferases
dc.subjectGalactosyltransferases
dc.subjectGlucosyltransferases
dc.subjectPolysaccharides
dc.subjectMannans
dc.subjectArabidopsis Proteins
dc.subjectPlant Mucilage
dc.titleThe Patterned Structure of Galactoglucomannan Suggests It May Bind to Cellulose in Seed Mucilage.
dc.typeArticle
prism.endingPage1026
prism.issueIdentifier3
prism.publicationDate2018
prism.publicationNamePlant Physiol
prism.startingPage1011
prism.volume178
dc.identifier.doi10.17863/CAM.32419
dcterms.dateAccepted2018-08-21
rioxxterms.versionofrecord10.1104/pp.18.00709
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
rioxxterms.licenseref.startdate2018-11
dc.contributor.orcidYu, Li [0000-0001-8820-6705]
dc.contributor.orcidLyczakowski, Jan J [0000-0002-7694-8629]
dc.contributor.orcidPereira, Caroline S [0000-0002-0248-0883]
dc.contributor.orcidKotake, Toshihisa [0000-0002-1167-3273]
dc.contributor.orcidMogelsvang, Soren [0000-0001-7415-8626]
dc.contributor.orcidSkaf, Munir S [0000-0001-7485-1228]
dc.contributor.orcidDupree, Paul [0000-0001-9270-6286]
dc.identifier.eissn1532-2548
rioxxterms.typeJournal Article/Review
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/L014130/1)
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/J014540/1)
pubs.funder-project-idLeverhulme Trust (RP2013-SL-008)
cam.issuedOnline2018-09-05
rioxxterms.freetoread.startdate2019-09-05


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